Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Effect of Peganum harmala Extract on Biofilm and Involved Gene Expression in Biofilm Production of Candida albicans

Fri Feb 23 13:17:10 2024

(2023) Effect of Peganum harmala Extract on Biofilm and Involved Gene Expression in Biofilm Production of Candida albicans. Jundishapur Journal of Microbiology. p. 8. ISSN 2008-3645

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Abstract

Background: Since common drug therapies cannot eradicate Candida biofilm, extensive studies are required to develop more ef-fective antifungal compounds and identify their mechanism of action against Candida biofilm. Peganum harmala L. is a traditional medicinal plant, the seeds of which have been used to treat various diseases. Objectives: This study aimed to investigate the anti-biofilm mechanisms of P. harmala extract (PHE) and the expression of CAT1, EFG1, and BCR1 genes involved in oxidative stress response and biofilm formation in Candida albicans. Methods: Anti-biofilm activity of PHE was evaluated by crystal violet assay to determine biofilm formation on 33 C. albicans isolates. Finally, a real-time polymerase chain reaction was performed to analyze the effect of PHE on the expression of CAT1, EFG1, and BCR1 genes in C. albicans. Results: This study determined the minimum biofilm eradication concentration (MBEC) of 15 isolates in concentrations between 0.49-3.9 mu g/mL of P. harmala extract. Statistical analysis showed that the exposure of C. albicans biofilm to PHE significantly reduced the expression of CAT1 mRNA in C. albicans isolates (P = 0.0068). However, no significant difference was observed in the expression of EFG1 and BCR1 genes. Conclusions: The results demonstrated that PHE significantly decreased CAT1 expression in C. albicans cells treated with the herbal extract. PHE is likely to accumulate hydrogen peroxide (H2O2) by reducing CAT1 expression and disrupting the pro-oxidant/antioxidant balance that leads to the overproduction of reactive oxygen species (ROS) and can cause damage to cellular components and eventually destroy C. albicans biofilm.

Item Type: Article
Creators:
CreatorsEmail
Erfaninejad, M.UNSPECIFIED
Aboualigalehdari, E.UNSPECIFIED
Fatahinia, M.UNSPECIFIED
Keywords: Biofilm Candida albicans CAT1 Gene Gene Expression Peganum harmala L. Superoxide Dismutase oxidative stress-response fluconazole Microbiology
Divisions:
Page Range: p. 8
Journal or Publication Title: Jundishapur Journal of Microbiology
Journal Index: ISI
Volume: 16
Number: 2
Identification Number: https://doi.org/10.5812/jjm-132692
ISSN: 2008-3645
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4410

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