Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Immune response induced by recombinant pres2/S-protein and a pres2-S-protein fused with a core 18-27 antigen fragment of hepatitis B virus compared to conventional HBV vaccine

Wed Feb 21 01:56:32 2024

(2023) Immune response induced by recombinant pres2/S-protein and a pres2-S-protein fused with a core 18-27 antigen fragment of hepatitis B virus compared to conventional HBV vaccine. Virus Genes. pp. 499-514. ISSN 0920-8569

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Abstract

Although comprehensive vaccination is the cornerstone of public health programs to control hepatitis B virus (HBV) infections, 5 of people who receive the existing vaccine do not develop proper immunity against HBV. To overcome this challenge, researchers have tried using various protein fragments encoded by the virus genome to achieve better immunization rates. An important antigenic component of HBsAg called the preS2/S or M protein has also received much attention in this area. The gene sequences of preS2/S and Core18-27 peptide were extracted from the GenBank (NCBI). Final gene synthesis was conducted with pET28. Groups of BALB/c mice were immunized with 10 mu g/ml of recombinant proteins and 1 mu g/ml CPG7909 adjuvant. Serum levels of IF-gamma, TNF-alpha, IL-2, IL-4, and IL-10 were measured by ELISA assay method on spleen cell cultures on day 45, and IgG1, IgG2a, and total IgG titers obtained from mice serum were quantified on days 14 and 45. Statistical analysis did not show any significant difference between the groups regarding IF-gamma level. There were, however, significant differences in terms of IL-2 and IL-4 levels between the groups receiving preS2/S-C18-27 with and without adjuvant and the groups receiving both preS2/S and preS2/S-C18-27 (Plus Recomb-Plus Recomb: the group of mice that received both preS2/S and preS2/S-C18-27 simultaneously). The strongest total antibody production was induced by immunization with both recombinant proteins without CPG adjuvant. The groups that received both preS2/S and preS2/S-C18-27, whether with or without adjuvant, were significantly different from those that received the conventional vaccine considering most abundant interleukins. This difference suggested that higher levels of efficacy can be achieved by the use of multiple virus antigen fragments rather than using a single fragment.

Item Type: Article
Creators:
CreatorsEmail
Parizad, E. G.UNSPECIFIED
Fooladi, A. A. I.UNSPECIFIED
Sedighian, H.UNSPECIFIED
Behzadi, E.UNSPECIFIED
Amani, J.UNSPECIFIED
Khosravi, A.UNSPECIFIED
Keywords: preS2HBsAg Hepatitis B virus Hepatitis B core antigen CpG 7909 Hepatitis B vaccines therapeutic vaccine surface-antigen cpg DNA protein hbsag gene immunogenicity epidemiology expression particles Genetics & Heredity Virology
Divisions:
Page Range: pp. 499-514
Journal or Publication Title: Virus Genes
Journal Index: ISI
Volume: 59
Number: 4
Identification Number: https://doi.org/10.1007/s11262-023-01995-z
ISSN: 0920-8569
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4390

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