Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Molecular Characterization of Resistance to Second-Line Anti-Mycobacterial Drugs among Clinical Isolates of Multidrug-Resistant Mycobacterium tuberculosis

Wed May 22 12:34:14 2024

(2023) Molecular Characterization of Resistance to Second-Line Anti-Mycobacterial Drugs among Clinical Isolates of Multidrug-Resistant Mycobacterium tuberculosis. Clinical Laboratory. p. 8. ISSN 1433-6510

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Background: The emergence of multidrug resistance and extensively drug-resistant tuberculosis is a serious public health crisis. Using rapid and inexpensive molecular methods such as HRM assay in the detection of second-line drugs resistance in M. tuberculosis would be helpful in the treatment and control of XDR tuberculosis cases. Methods: MDR-TB isolates were collected from Iranian tuberculosis laboratories. Drug susceptibility test performed via the indirect proportion method utilizing LJ Medium. Susceptibility to ciprofloxacin, ofloxacin, amikacin, kanamycin, and capreomycin, as second-line anti-tuberculosis agents were assessed. Single point mutations in gyrA, rrs and eis genes were detected via HRM assay and DNA sequencing. Results: A DST test was performed for 56 MDR isolates and at least 27 (48.2) isolates were resistant to CIP or OFL. Also, 14 (25), 12 (21.4), and 15 (26.7) isolates were resistant to capreomycin, amikacin, and kanamycin, respectively. D94G, A90V, and G88C mutations were the most frequent mutations in gyrA gene. Also, A1401G mutation was detected more than the other mutations in rrs gene. Conclusions: The frequency of CIP/OFL and AMK/CAP/KAN-resistant TB is considerable among Iranian tuberculosis cases. HRM assay is a rapid and inexpensive test and can detect important mutation-based drug resistance in MDR-TB and XDR-TB isolates.

Item Type: Article
Creators:
CreatorsEmail
Habibnia, S.UNSPECIFIED
Karami-Zarandi, M.UNSPECIFIED
Zaker, S.UNSPECIFIED
Ghalavand, Z.UNSPECIFIED
Doustdar, F.UNSPECIFIED
Eslami, G.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Keywords: MDR-TB XDR-TB HRM M tuberculosis rrs a1401g mutation fluoroquinolone resistance capreomycin resistance amikacin kanamycin genes gyrb Medical Laboratory Technology
Divisions:
Page Range: p. 8
Journal or Publication Title: Clinical Laboratory
Journal Index: ISI
Identification Number: https://doi.org/10.7754/Clin.Lab.2022.220211
ISSN: 1433-6510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4366

Actions (login required)

View Item View Item