Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Controlling the Heterogeneous Vancomycin Intermediated Staphylococcus aureus (hVISA) Through the Use of Rosmarinus officinalis L. Leaves Extract

Mon Apr 15 05:47:54 2024

(2022) Controlling the Heterogeneous Vancomycin Intermediated Staphylococcus aureus (hVISA) Through the Use of Rosmarinus officinalis L. Leaves Extract. Karbala International Journal of Modern Science. pp. 640-650. ISSN 2405609X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Treatment failure and persistent infection are two serious problems that result from Staphylococcus aureus having decreased vancomycin susceptibility. The present study investigates the effects of Rosmarinus officinalis L. leaves extract on the growth and quorum sensing (QS) of heterogeneous vancomycin-intermediate S. aureus hVISA isolates. Real-time RT-polymerase chain reaction (PCR) is used to determine the transcriptional changes of the accessory gene regulator (agr) in the (hVISA) strain after and before being treated with the R. officinalis leaves extract. The antibacterial activity of the EOs was determined through the use of a microtitre plate test (MtP); with a minimum inhibitory concentration (MIC) of 0.7 mg/ml against hVISA, a rosemary leaves extract has a high level of inhibitory activity. However, the extraction of essential oils is done in a soxhlet apparatus by using ethanol 96. Gas chromatography and mass spectrometry (GC/MS) and revealed the most common chemicals containing verbenone, 36.20, and 1,8-cineol (Eucalyptol), 12.14. Finally, after treatment with sub-MIC concentration, hld gene levels significantly decreased in hVISA strains (a mean of 0.4-fold) in comparison to the control strains (1.0-fold) in late-exponential phases, regardless of whether the agr mutation is present. The down regulation of the hld gene may be a significant genetic event in the hVISA strain. © 2022 University of Kerbala.

Item Type: Article
Creators:
CreatorsEmail
Al-Hayali, O. Z.UNSPECIFIED
Al-Marjani, M. F.UNSPECIFIED
Maleki, A.UNSPECIFIED
Keywords: Antibacterial activity Antimicrobial activity Chemical composition Real-time PCR
Divisions:
Page Range: pp. 640-650
Journal or Publication Title: Karbala International Journal of Modern Science
Journal Index: Scopus
Volume: 8
Number: 4
Identification Number: https://doi.org/10.33640/2405-609X.3264
ISSN: 2405609X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4311

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