Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture

Tue Feb 27 21:03:32 2024

(2022) Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture. Basic and Clinical Neuroscience. pp. 625-636. ISSN 2008126X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Introduction: The induction of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs into dopaminergic neuron-like cells. Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuronlike cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography. Results: Our results showed that dopaminergic neuronal markers' transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates. Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases. © 2022 Iran University of Medical Sciences. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Moayeri, A.UNSPECIFIED
Alizadeh, R.UNSPECIFIED
Hamidabadi, H. G.UNSPECIFIED
Bojnordi, M. N.UNSPECIFIED
Niapour, A.UNSPECIFIED
Hedayatpour, A.UNSPECIFIED
Darvishi, M.UNSPECIFIED
Heidari, F.UNSPECIFIED
Solemani, M.UNSPECIFIED
Elyasi, L.UNSPECIFIED
Keywords: Differentiation Dopaminergic neurons Human umbilical cord mesenchymal stem cells In vitro matrigel Article cell culture cell differentiation cell transdifferentiation controlled study dopamine release dopaminergic nerve cell flow cytometry genetic transcription high performance liquid chromatography histogram human human cell immunocytochemistry immunofluorescence MTT assay nerve cell differentiation real time polymerase chain reaction umbilical cord mesenchymal stem cell
Divisions:
Page Range: pp. 625-636
Journal or Publication Title: Basic and Clinical Neuroscience
Journal Index: Scopus
Volume: 13
Number: 5
Identification Number: https://doi.org/10.32598/bcn.2021.973.3
ISSN: 2008126X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4300

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