Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

In silico design of a novel peptide-based vaccine against the ubiquitous apicomplexan Toxoplasma gondii using surface antigens

Thu Jul 25 22:10:38 2024

(2023) In silico design of a novel peptide-based vaccine against the ubiquitous apicomplexan Toxoplasma gondii using surface antigens. In silico pharmacology. p. 5. ISSN 2193-9616 (Print) 2193-9616 (Electronic) 2193-9616 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/36960094

Abstract

Human toxoplasmosis is a global public health concern and a commercial vaccine is still lacking. The present in silico study was done to design a novel vaccine candidate using tachyzoite-specific SAG1-realted sequence (SRS) proteins. Overlapping B-cell and strictly-chosen human MHC-I binding epitopes were predicted and connected together using appropriate spacers. Moreover, a TLR4 agonist, human high mobility group box protein 1 (HMGB1), and His-tag were added to the N- and C-terminus of the vaccine sequence. The final vaccine had 442 residues and a molecular weight of 47.71 kDa. Physico-chemical evaluation showed a soluble, highly antigenic and non-allergen protein, with coils and helices as secondary structures. The vaccine 3D model was predicted by ITASSER server, subsequently refined and was shown to possess significant interactions with human TLR4. As well, potent stimulation of cellular and humoral immunity was demonstrated upon chimeric vaccine injection. Finally, the outputs showed that this vaccine model possesses top antigenicity, which could provoke significant cell-mediated immune profile including IFN-gamma, and can be utilized towards prophylactic purposes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40203-023-00140-w.

Item Type: Article
Creators:
CreatorsEmail
Shams, M.UNSPECIFIED
Heydaryan, S.UNSPECIFIED
Bashi, M. C.UNSPECIFIED
Gorgani, B. N.UNSPECIFIED
Ghasemi, E.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Nazari, N.UNSPECIFIED
Irannejad, H.UNSPECIFIED
Keywords: Multi-epitope vaccine SRS proteins Tachyzoite Toxoplasma gondii Vaccinology
Divisions:
Page Range: p. 5
Journal or Publication Title: In silico pharmacology
Journal Index: Pubmed
Volume: 11
Number: 1
Identification Number: https://doi.org/10.1007/s40203-023-00140-w
ISSN: 2193-9616 (Print) 2193-9616 (Electronic) 2193-9616 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4254

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