Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Effects of human chorionic gonadotropin-producing peripheral blood mononuclear cells on the endometrial receptivity and implantation sites of the mouse uterus

Fri Jul 19 02:28:50 2024

(2022) Effects of human chorionic gonadotropin-producing peripheral blood mononuclear cells on the endometrial receptivity and implantation sites of the mouse uterus. Clinical and Experimental Reproductive Medicine-Cerm. pp. 248-258. ISSN 2233-8233

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Official URL: <Go to ISI>://WOS:000892528800004

Abstract

Objective: This research investigated the effects of human chorionic gonadotropin (HCG)-producing peripheral blood mononuclear cells (PBMCs) on the implantation rate and embryo attachment in mice. Methods: In this experimental study, a DNA fragment of the HCG gene was cloned into an expression vector, which was transfected into PB-MCs. The concentration of the produced HCG was measured using enzyme-linked immunosorbent assay. Embryo attachment was investi-gated on the co-cultured endometrial cells and PBMCs in vitro. As an in vivo experiment, intrauterine administration of PBMCs was done in plaque-positive female mice. Studied mice were distributed into five groups: control, embryo implantation dysfunction (EID), EID with pro-duced HCG, EID with PBMCs, and EID with HCG-producing PBMCs. Uterine horns were excised to characterize the number of implantation sites and pregnancy rate on day 7.5 post-coitum. During an implantation window, the mRNA expression of genes was evaluated using re-al-time polymerase chain reaction.Results: DNA fragments were cloned between the BamHI and EcoRI sites in the vector. About 465 pg/mL of HCG was produced in the trans-fected PBMCs. The attachment rate, pregnancy rate, and the number of implantation sites were substantially higher in the HCG-producing PBMCs group than in the other groups. Significantly elevated expression of the target genes was observed in the EID with HCG-producing PBMCs group.Conclusion: Alterations in gene expression following the intrauterine injection of HCG-producing PBMCs, could be considered a possible cause of increased embryo attachment rate, pregnancy rate, and the number of implantation sites.

Item Type: Article
Creators:
CreatorsEmail
Rezaee, D.UNSPECIFIED
Bandehpour, M.UNSPECIFIED
Kazemi, B.UNSPECIFIED
Hosseini, S.UNSPECIFIED
Dehghan, Z.UNSPECIFIED
Bastaminejad, S.UNSPECIFIED
Salehi, M.UNSPECIFIED
Keywords: Embryo implantation Human chorionic gonadotropin Infertility Peripheral blood mononuclear cell embryo-transfer cycles pregnancy rates immune-system in-vitro improves failure pbmc Obstetrics & Gynecology Reproductive Biology
Divisions:
Page Range: pp. 248-258
Journal or Publication Title: Clinical and Experimental Reproductive Medicine-Cerm
Journal Index: ISI
Volume: 49
Number: 4
Identification Number: https://doi.org/10.5653/cerm.2022.05358
ISSN: 2233-8233
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4236

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