Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Computational Clues of Immunogenic Hotspots in Plasmodium falciparum Erythrocytic Stage Vaccine Candidate Antigens: In Silico Approach

Sun Apr 21 15:09:22 2024

(2022) Computational Clues of Immunogenic Hotspots in Plasmodium falciparum Erythrocytic Stage Vaccine Candidate Antigens: In Silico Approach. Biomed Research International. p. 21. ISSN 2314-6133

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Official URL: <Go to ISI>://WOS:000876511300004

Abstract

Malaria is the most pernicious parasitic infection, and Plasmodium falciparum is the most virulent species with substantial morbidity and mortality worldwide. The present in silico investigation was performed to reveal the biophysical characteristics and immunogenic epitopes of the 14 blood-stage proteins of the P. falciparum using comprehensive immunoinformatics approaches. For this aim, various web servers were employed to predict subcellular localization, antigenicity, allergenicity, solubility, physicochemical properties, posttranslational modification sites (PTMs), the presence of signal peptide, and transmembrane domains. Moreover, structural analysis for secondary and 3D model predictions were performed for all and stable proteins, respectively. Finally, human helper T lymphocyte (HTL) epitopes were predicted using HLA reference set of IEDB server and screened in terms of antigenicity, allergenicity, and IFN-gamma induction as well as population coverage. Also, a multiserver B-cell epitope prediction was done with subsequent screening for antigenicity, allergenicity, and solubility. Altogether, these proteins showed appropriate antigenicity, abundant PTMs, and many B-cell and HTL epitopes, which could be directed for future vaccination studies in the context of multiepitope vaccine design.

Item Type: Article
Creators:
CreatorsEmail
Azimi-Resketi, M.UNSPECIFIED
Heydaryan, S.UNSPECIFIED
Kumar, N.UNSPECIFIED
Takalou, A.UNSPECIFIED
Dizaji, R. E.UNSPECIFIED
Gorgani, B. N.UNSPECIFIED
Shams, M.UNSPECIFIED
Keywords: apical membrane antigen-1 b-cell epitopes merozoite antigens malaria protein prediction antibody immunization invasion binding Biotechnology & Applied Microbiology Research & Experimental Medicine
Divisions:
Page Range: p. 21
Journal or Publication Title: Biomed Research International
Journal Index: ISI
Volume: 2022
Identification Number: https://doi.org/10.1155/2022/5886687
ISSN: 2314-6133
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4199

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