Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Amoxicillin-docosahexaenoic acid encapsulated chitosan-alginate nanoparticles as a delivery system with enhanced biocidal activities against Helicobacter pylori and improved ulcer healing

Tue Apr 16 08:13:41 2024

(2023) Amoxicillin-docosahexaenoic acid encapsulated chitosan-alginate nanoparticles as a delivery system with enhanced biocidal activities against Helicobacter pylori and improved ulcer healing. Frontiers in Microbiology. p. 13.

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Official URL: <Go to ISI>://WOS:000936152400001

Abstract

Encapsulation of amoxicillin (AMX) for drug delivery against Helicobacter pylori infection and aspirin-induced ulcers in rat's stomachs was performed using docosahexaenoic acid (DHA)-loaded chitosan/alginate (CA) nanoparticles (NPs) developed by ionotropic gelation method. The physicochemical analyses of the composite NPs were performed by scanning electron microscopy, Fourier transform infrared spectroscopy, zeta potential, X-ray diffraction, and atomic force microscopy. The encapsulation efficiency of AMX was increased to 76 by incorporating DHA, which resulted in a reduction in the particle size. The formed CA-DHA-AMX NPs effectively adhered to the bacteria and rat gastric mucosa. Their antibacterial properties were more potent than those of the single AMX and CA-DHA NPs as demonstrated by the in vivo assay. The composite NPs attained higher mucoadhesive potential during food intake than during fasting (p = 0.029). At 10 and 20 mg/kg AMX, the CA-AMX-DHA showed more potent activities against H. pylori than the CA-AMX, CA-DHA, and single AMX. The in vivo study showed that the effective dose of AMX was lower when DHA was included, indicating better drug delivery and stability of the encapsulated AMX. Both mucosal thickening and ulcer index were significantly higher in the groups receiving CA-DHA-AMX than in the groups receiving CA-AMX and single AMX. The presence of DHA declines the pro-inflammatory cytokines including IL-1 beta, IL-6, and IL-17A. The synergistic effects of AMX and the CA-DHA formulation increased the biocidal activities against H. pylori infection and improved ulcer healing properties.

Item Type: Article
Creators:
CreatorsEmail
Khoshnood, S.UNSPECIFIED
Negahdari, B.UNSPECIFIED
Kaviar, V. H.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Abdullah, M. A.UNSPECIFIED
El-Shazly, M.UNSPECIFIED
Haddadi, M. H.UNSPECIFIED
Keywords: chitosan-DHA nanoparticles Helicobacter pylori biocidal activities amoxicillin encapsulation chitosan docosahexaenoic antibacterial activity mechanism infection ph Microbiology
Divisions:
Page Range: p. 13
Journal or Publication Title: Frontiers in Microbiology
Journal Index: ISI
Volume: 14
Identification Number: https://doi.org/10.3389/fmicb.2023.1083330
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4190

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