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Ilam University of Medical Sciences

Microbiological and drug resistance patterns of bronchoalveolar lavage samples taken from hospitalized patients in Iran

Fri Apr 12 19:33:11 2024

(2022) Microbiological and drug resistance patterns of bronchoalveolar lavage samples taken from hospitalized patients in Iran. Germs. pp. 333-343. ISSN 2248-2997

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Official URL: <Go to ISI>://WOS:000914432600002

Abstract

Introduction Pulmonary diseases are amongst the most common causes of premature death and distressing disorders worldwide. This study aimed to detect the fastidious and routine infectious agents, and their drug resistance patterns in bronchoalveolar lavage (BAL) samples.Methods A total of 44 BAL samples were collected by bronchoscopy from patients with respiratory disorders hospitalized at 2 teaching hospitals in Ilam, Iran. The samples were cultured on routine bacterial culture media to identify the bacterial agents and calculate the colony count. Antibiotic susceptibility was determined by disk diffusion method according to the CLSI protocol. PCR was used to detect the fastidious bacteria Mycoplasma pneumoniae and Chlamydia pneumoniae using the 16srRNA specific primers and Legionella pneumophila using the mip specific primers.Results Overall, 100 bacterial isolates were isolated by culture from the 44 BAL samples including: Staphylococcus aureus (24, 31.2 ), Streptococcus pyogenes (18, 23.4 ), Enterococcus spp. (11, 14.3 ), Acinetobacter baumannii (11, 14.3 ), Pseudomonas aeruginosa (11, 14.3 ), Enterococcus spp. (10, 13), Micrococcus spp. (5, 6.5 ), Staphylococcus epidermidis (5, 6.5) and Klebsiella pneumoniae (5, 6.5). PCR detected 4 positive samples (9.1 ) for Chlamydia pneumoniae but no positive cases for Mycoplasma pneumoniae and Legionella pneumophila. Acinetobacter baumannii showed the highest resistance rate (81.8 ) to aztreonam and ceftazidime. Seventy-five percent of the Staphylococcus aureus isolates were resistant to cefoxitin (MRSA) and 83.3 had the mecA gene. Vancomycin resistance was observed in 27.3 of the Enterococcus species (VRE). Resistance to piperacillin, cefotaxime, ciprofloxacin and imipenem was observed in 54.5, 45.5, and 36.4 of the Pseudomonas aeruginosa isolates, respectively. The frequency of organisms isolated from the ICU was higher (46) than from other wards.Conclusions The presence of MRSA, cephalosporins-resistant Enterobacteriaceae as well as Pseudomonas aeruginosa and Acinetobacter baumannii resistant against piperacillin, imipenem, cefotaxime, aztreonam and ciprofloxacin amongst different wards, especially the ICU ward of the surveyed hospitals, is a major healthcare concern and it is necessary to wisely scrutinize the preventive strategies for antibiotic resistant infections.

Item Type: Article
Creators:
CreatorsEmail
Tahmasebi, Z.UNSPECIFIED
Asadollahi, P.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Kalaei, E. G. P.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Keywords: Bronchoalveolar lavage antibiotic resistance ICU hospital PCR susceptibility infections oxacillin pneumonia Infectious Diseases
Divisions:
Page Range: pp. 333-343
Journal or Publication Title: Germs
Journal Index: ISI
Volume: 12
Number: 3
Identification Number: https://doi.org/10.18683/germs.2022.1337
ISSN: 2248-2997
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4188

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