Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of Pseudomonas aeruginosa isolated from burn wound infection

Tue Jul 23 03:27:11 2024

(2023) Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of Pseudomonas aeruginosa isolated from burn wound infection. Journal of Clinical Laboratory Analysis. p. 9. ISSN 0887-8013

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Official URL: <Go to ISI>://WOS:000937556900001

Abstract

BackgroundBurn injuries result in disruption of the skin barrier against opportunistic infections. Pseudomonas aeruginosa is one of the main infectious agents colonizing burn wounds and making severe infections. Biofilm production and other virulence factors along with antibiotic resistance limit appropriate treatment options and time. Materials and MethodsWound samples were collected from hospitalized burn patients. P. aeruginosa isolates and related virulence factors identified by the standard biochemical and molecular methods. Antibiotic resistance patterns were determined by the disc diffusion method and beta-lactamase genes were detected by polymerase chain reaction (PCR) assay. To determine the genetic relatedness amongst the isolates, enterobacterial repetitive intergenic consensus (ERIC)-PCR was also performed. ResultsForty P. aeruginosa isolates were identified. All of these isolates were biofilm producers. Carbapenem resistance was detected in 40 of the isolates, and bla(TEM) (37/5), bla(VIM) (30), and bla(CTX-M) (20) were the most common beta-lactamase genes. The highest resistance was detected to cefotaxime, ceftazidime, meropenem, imipenem and piperacillin, and 16 (40) isolates were resistant to these antibiotics. The minimum inhibitory concentrations (MIC) of colistin was lower than 2 mu g/mL and no resistance was observed. Isolates were categorized to 17 MDR, 13 mono-drug resistance, and 10 susceptible isolates. High genetic diversity was also observed among the isolates (28 ERIC types) and most carbapenem-resistant isolates were classified into four main types. ConclusionAntibiotic resistance, particularly carbapenem resistance was considerable among the P. aeruginosa isolates colonizing burn wounds. Combining carbapenem resistance with biofilm production and virulence factors would result in severe and difficult-to-treat infections.

Item Type: Article
Creators:
CreatorsEmail
Ghasemian, S.UNSPECIFIED
Karami-Zarandi, M.UNSPECIFIED
Heidari, H.UNSPECIFIED
Khoshnood, S.UNSPECIFIED
Kouhsari, E.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Maleki, A.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Keywords: biofilm burn injuries carbapenem-resistant ERIC-PCR Pseudomonas aeruginosa strains genes prevalence Medical Laboratory Technology
Divisions:
Page Range: p. 9
Journal or Publication Title: Journal of Clinical Laboratory Analysis
Journal Index: ISI
Volume: 37
Number: 4
Identification Number: https://doi.org/10.1002/jcla.24850
ISSN: 0887-8013
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4186

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