Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Antimicrobial resistance and biofilm formation capacity among Acinetobacter baumannii strains isolated from patients with burns and ventilator-associated pneumonia

Fri Apr 12 17:56:49 2024

(2023) Antimicrobial resistance and biofilm formation capacity among Acinetobacter baumannii strains isolated from patients with burns and ventilator-associated pneumonia. Journal of Clinical Laboratory Analysis. p. 10. ISSN 0887-8013

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Official URL: <Go to ISI>://WOS:000904006700001

Abstract

BackgroundAcinetobacter baumannii is a pathogen responsible for nosocomial infections, especially in patients with burns and ventilator-associated pneumonia (VAP). The aims of this study was to compare the biofilm formation capacity, antimicrobial resistance patterns and molecular typing based on PFGE (Pulsed-Field Gel Electrophoresis) in A. baumannii isolated from burn and VAP patients. Materials and MethodsA total of 50 A. baumannii isolates were obtained from burn and VAP patients. In this study, we assessed antimicrobial susceptibility, biofilm formation capacity, PFGE fingerprinting, and the distribution of biofilm-related genes (csuD, csuE, ptk, ataA, and ompA). ResultsOverall, 74 of the strains were multidrug resistant (MDR), and 26 were extensively drug-resistant (XDR). Regarding biofilm formation capacity, 52, 36, and 12 of the isolates were strong, moderate, and weak biofilm producers. Strong biofilm formation capacity significantly correlated with XDR phenotype (12/13, 92.3). All the isolates harbored at least one biofilm-related gene. The most prevalent gene was csuD (98), followed by ptk (90), ataA (88), ompA (86), and csuE (86). Harboring all the biofilm-related genes was significantly associated with XDR phenotype. Finally, PFGE clustering revealed 6 clusters, among which cluster No. 2 showed a significant correlation with strong biofilm formation and XDR phenotype. ConclusionOur findings revealed the variable distribution of biofilm-related genes among MDR and XDR A. baumannii isolates from burn and VAP patients. A significant correlation was found between strong biofilm formation capacity and XDR phenotype. Finally, our results suggested that XDR phenotype was predominant among strong-biofilm producer A. baumannii in our region.

Item Type: Article
Creators:
CreatorsEmail
Khoshnood, S.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Mahdian, N.UNSPECIFIED
Heidary, M.UNSPECIFIED
Mahdian, S.UNSPECIFIED
Mohammadi, M.UNSPECIFIED
Maleki, A.UNSPECIFIED
Haddadi, M. H.UNSPECIFIED
Keywords: Acinetobacter baumannii antimicrobial resistance biofilm biofilm-related genes PFGE field gel-electrophoresis pseudomonas-aeruginosa rep-pcr genes infection virulence pattern Medical Laboratory Technology
Divisions:
Page Range: p. 10
Journal or Publication Title: Journal of Clinical Laboratory Analysis
Journal Index: ISI
Volume: 37
Number: 1
Identification Number: https://doi.org/10.1002/jcla.24814
ISSN: 0887-8013
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4167

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