Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Immobilization of Clostridium perfringens type D in calcium alginate beads: toxin production mimics free cell culture

Wed May 22 11:48:30 2024

(2022) Immobilization of Clostridium perfringens type D in calcium alginate beads: toxin production mimics free cell culture. Iranian Journal of Microbiology. pp. 503-509. ISSN 20083289 (ISSN)

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Background and Objectives: Cell-immobilization is used to maintain microbial culture to produce metabolites in repeat-ed-batch or continuous fermentations, thereby reducing the time and resources spent on delivering mass production of microbe. The technique also enables shortening of the detoxification phase and the amount of formaldehyde required due to low incidence of viable bacteria in the extract. Materials and Methods: A solution of sodium alginate containing Clostridium perfringens cells was dropped into stirring CaCl solution via a sterile syringe needle. Optimizations resulted in reasonably uniform beads containing C. perfringens. 2 Beads were externally stabilized by poly L-lysine, followed by immersion in a solution of Na-alginate to coat them with a new layer of alginate forming an alginate-PLL-alginate cortex. Results: This study proved successful in immobilizing C. perfringens cells inside uniform alginate microspheres. Cell load-ing and cell propagation inside the beads were measured. The cell loaded beads were cultivable in liquid media producing 550 minimum lethal doses per milliliter (MLD/ml) in a 72 h. Conclusion: The research paved the way for further investigations to optimize and establish an efficient bacterial encapsulation method. Thus, it seems possible to produce toxins from beads engulfing C. perfringens on larger scales via repeat-ed-batch or continuous fermentation processes. © 2022 The Authors. Published by Tehran University of Medical Sciences.

Item Type: Article
Rakhshandeh, H.UNSPECIFIED
Familsatarian, B.UNSPECIFIED
Nooshadokht, M.UNSPECIFIED
Amirheidari, B.UNSPECIFIED
Keywords: Calcium alginate beads Cell-immobilization techniques Clostridium perfringens Encapsulation Toxin production alginic acid calcium alginate calcium chloride cysteine disodium hydrogen phosphate formaldehyde lysine microsphere soy peptone toxin unclassified drug yeast extract analytical parameters Article bacterial proliferation bacterium culture bead shape bead size bead stability bead stabilization cell culture cell propagation Clostridium perfringens type D controlled study culture optimization detoxification phase immersion immobilized cell membrane stabilization microbial loading minimum lethal dose nonhuman physical property physical strength reaction optimization resilience uniformity
Page Range: pp. 503-509
Journal or Publication Title: Iranian Journal of Microbiology
Journal Index: Scopus
Volume: 14
Number: 4
Identification Number:
ISSN: 20083289 (ISSN)
Depositing User: مهندس مهدی شریفی

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