Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Distribution and expression of virulence genes (hlyA, sat) and genotyping of Escherichia coli O25b/ST131 by multi-locus variable number tandem repeat analysis in Tehran, Iran

Mon Mar 4 07:38:29 2024

(2022) Distribution and expression of virulence genes (hlyA, sat) and genotyping of Escherichia coli O25b/ST131 by multi-locus variable number tandem repeat analysis in Tehran, Iran. Acta microbiologica et immunologica Hungarica. ISSN 1588-2640 (Electronic) 1217-8950 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/36129793

Abstract

Escherichia coli ST131 is a pandemic clone with high antibiotic resistance, and it is a major causative agent of urinary tract infection (UTI) and bloodstream infections. This study evaluated the distribution and expression of virulence genes and genotyping of E. coli O25b/ST131 by Multi-locus variable number tandem repeat analysis (MLVA) method among UTI in patients at Tehran hospitals, Iran. A total of 107 E. coli isolates were collected from UTI patients. Polymerase chain reaction (PCR) amplification of the pabB gene was used to identify E. coli O25b/ST131 and the prevalence of sat and hlyA virulence genes was also analyzed. The microtiter method quantified biofilm formation ability in E. coli O25b/ST131. The Real-Time PCR (qRT-PCR) was performed to evaluate the expression of sat and hlyA genes. Finally, MLVA was performed for E. coli O25b/ST131 genotyping by targeting seven tandem repeats. SPSS-16 software was used for statistical analysis. Molecular study showed that 71 of isolates carried the pabB gene and were considered E. coli O25b/ST131 strains. Also, 45.8 and 17.8 of isolates carried sat and hlyA genes, respectively. The 57.9 isolates had biofilm formation ability. Expression of the studied virulence genes showed an increase in strong biofilm producing E. coli O25b/ST131 strains. A total of 76 (100) E. coli O25b/ST131 strains were typed by the MLVA method. High prevalence of E. coli O25b/ST131 isolates in UTI patients can be a serious warning to the treatment due to the high antibiotic resistance rate, expression of virulence genes, and biofilm formation.

Item Type: Article
Creators:
CreatorsEmail
Asgharzadeh, S.UNSPECIFIED
Golmoradi Zadeh, R.UNSPECIFIED
Taati Moghadam, M.UNSPECIFIED
Farahani Eraghiye, H.UNSPECIFIED
Sadeghi Kalani, B.UNSPECIFIED
Masjedian Jazi, F.UNSPECIFIED
Mirkalantari, S.UNSPECIFIED
Keywords: E. coli ST131 Mlva biofilm production urinary tract infection
Divisions:
Journal or Publication Title: Acta microbiologica et immunologica Hungarica
Journal Index: Pubmed
Identification Number: https://doi.org/10.1556/030.2022.01826
ISSN: 1588-2640 (Electronic) 1217-8950 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4063

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