Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Ethanol and Cancer Induce Similar Changes on Protein Expression Pattern of Human Fibroblast Cell

Wed Jun 19 02:04:45 2024

(2016) Ethanol and Cancer Induce Similar Changes on Protein Expression Pattern of Human Fibroblast Cell. Iranian Journal of Pharmaceutical Research. pp. 175-184. ISSN 1735-0328

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Ethanol has a vast consumption around the world. Many researches confirmed some adverse effect of this component on human health. In addition, recent studies showed significant alteration in both cellular population, and protein profile of human foreskin fibroblast cell line (HFFF2) in the specific dosage of ethanol. Here, the role and interaction of some proteins (characterized by significant alteration in expression due to ethanol effect) analyzed by proteomics and evaluated by considering cancerous case. 2D-electrophoresis findings of comparison of normal fibroblast cells and treated fibroblast with 270 mM dosage of ethanol analyzed by using SameSpots software, R software, and Cytoscape for protein-protein interaction (PPI) investigation. Six proteins with significantly altered expression associated with fundamental properties in a cell identified in ethanol-treated sample. These include AnnexinA5, Heterogeneous nuclear ribonucleoprotein A1, Rho-GDP dissociation inhibitor, Cathepsin L, Cu/Zn-SOD, Rho-GDP dissociation inhibitor, and Serpin peptidase inhibitor. Surprisingly, all these proteins were down-regulated and this pattern is similar to nasopharyngeal carcinoma-associated stromal fibroblast sample. Additionally, protein-protein interaction (PPI) indicates that HNRNPA1, SERPINE1 are hub proteins. Once their expression alters, it can impose vast changes on other molecular function. Based on this approach, ethanol may target same pathways that are related to cancer onset. In addition, some epidemiologic studies proved that ethanol consumption is related to increment of cancer risk. Therefore, more investigation is required in this regard to elicit the feasible relationship.

Item Type: Article
Zamanian-Azodi, M.UNSPECIFIED
Rezaei-Tavirani, M.UNSPECIFIED
Keywords: Ethanol Human Foreskin Fibroblast cell line (HFFF2) Proteomics Gene Ontology Protein-protein Interaction (PPIs) plasminogen-activator inhibitor-1 gdp-dissociation inhibitor human breast-cancer superoxide-dismutase alcohol-consumption cathepsin-l colorectal-cancer lung-cancer cu/zn-sod disease Pharmacology & Pharmacy
Page Range: pp. 175-184
Journal or Publication Title: Iranian Journal of Pharmaceutical Research
Journal Index: ISI
Volume: 15
ISSN: 1735-0328
Depositing User: مهندس مهدی شریفی

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