Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Towards the First Multiepitope Vaccine Candidate against Neospora caninum in Mouse Model: Immunoinformatic Standpoint

Fri Apr 12 18:46:56 2024

(2022) Towards the First Multiepitope Vaccine Candidate against Neospora caninum in Mouse Model: Immunoinformatic Standpoint. Biomed Research International. p. 15. ISSN 2314-6133

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Abstract

Neospora caninum is an economically significant parasite among livestock, particularly in dairy cattle herds, causing storm abortions. Vaccination seems necessary to limit the infection and its harsh consequences. This is the first steps towards developing a multiepitope vaccine candidate against N. caninum using in silico approaches. High-ranked mouse MHC-binding and shared linear B-cell epitopes from six proteins (SRS2, MIC3, MIC6, GRA1, IMP-1, and profilin) as well as IFN-gamma-inducing epitopes (from SAG1) were predicted, screened, and connected together through appropriate linkers. Finally, RS-09 protein (TLR4 agonist) and histidine tag were added to N- and C-terminal of the vaccine sequence, yielding 486 residues in length. Physicochemical properties showed a stable (instability index: 27.23), highly soluble, antigenic (VaxiJen score: 0.9554), and nonallergenic candidate. Secondary structure of the multiepitope protein included 58.85 random coil, 20.99 extended strand, and 20.16 alpha helix. Also, the tertiary structure was predicted, and further analyses validated a stable interaction between the vaccine model and mouse TLR4 (binding score: -1261.6). Virtual simulation of immune profile demonstrated potently stimulated humoral (IgG+IgM) and cell-mediated (IFN-gamma) responses upon multiepitope vaccine injection. Altogether, a potentially immunogenic vaccine candidate was developed using several N. caninum proteins, with the capability to elicit IFN-gamma upsurge and other components of cellular immunity, and can be used in prophylactic purposes against neosporosis.

Item Type: Article
Creators:
CreatorsEmail
Shams, M.UNSPECIFIED
Maleki, B.UNSPECIFIED
Kordi, B.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Nazari, N.UNSPECIFIED
Irannejad, H.UNSPECIFIED
Asghari, A.UNSPECIFIED
Keywords: host-parasite relationship b-cell epitopes definitive hosts infection prediction proteins cattle acid mice web Biotechnology & Applied Microbiology Research & Experimental Medicine
Divisions:
Page Range: p. 15
Journal or Publication Title: Biomed Research International
Journal Index: ISI
Volume: 2022
Identification Number: https://doi.org/10.1155/2022/2644667
ISSN: 2314-6133
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4026

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