Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Novel naltrexone hydrochloride nanovaccine based on chitosan nanoparticles promotes induction of Th1 and Th17 immune responses resulting in protection against Toxoplasma gondii tachyzoites in a mouse model

Mon Jul 22 15:33:15 2024

(2022) Novel naltrexone hydrochloride nanovaccine based on chitosan nanoparticles promotes induction of Th1 and Th17 immune responses resulting in protection against Toxoplasma gondii tachyzoites in a mouse model. International Journal of Biological Macromolecules. pp. 962-972. ISSN 0141-8130

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Abstract

This study was aimed to encapsulate and construct the Toxoplasma gondii surface antigen (SAG1) and naltrexone hydrochloride (NLT-HCL) as an adjuvant within chitosan nanoparticles (CS-NPs) to develop efficacious vaccine against T. gondii. Seven groups of BALB/c mice were immunized with SAG1, chitosan (CS), NLT-SAG1, CS-SAG1, CS-SAG1-NLT, CS-NLT and PBS. The efficiency of each approach was detected in vivo mouse immunization. Moreover, the immuno-induction effect of SAG1 recombinant protein and CS-NPs-based NLT-HCL as an adjuvant in a vaccine delivery was evaluated. Experimentally, Th1/Th17 biased cellular and humoral immune responses were activated in the mice immunized with CS-SAG1-NLT nanoparticles that were accompanied by considerable increased production of IFN-gamma, IL-17, IL-12, IL-4, IFN-gamma/IL-4 ratio, IgG, IgG2a. This group of mice also showed significantly increased survival time post-challenging. The successful encapsulated SAG1 recombinant protein and NLT-HCL, as an adjuvant, within CS-NPs can induce immune responses against toxoplasmosis. We could incorporate NLT-HCL adjuvant into the CS-NPs based delivery systems, which makes CS-NPs attractive as a colloidal carrier system for NLT-HCL as secondary adjuvant. This new approach or the simultaneous use of CS and NLT demonstrated that the co-administration of CS-NPs and NLT-HCL induce production of IL-17 cytokine. This approach can be used for vaccination purposes, in which Th17 and Th1 cellular immune are considered the key of the successful immune response.

Item Type: Article
Creators:
CreatorsEmail
Khorshidvand, Z.UNSPECIFIED
Khosravi, A.UNSPECIFIED
Mahboobian, M. M.UNSPECIFIED
Larki-Harchegani, A.UNSPECIFIED
Fallah, M.UNSPECIFIED
Maghsood, A. H.UNSPECIFIED
Keywords: Nanoparticles Chitosan Adjuvant Naltrexone Toxoplasma gondii SAG1 opioid antagonist naloxone vaccine adjuvants receptor antagonist delivery-systems antigen alum elicits mixture leishmaniasis propranolol Biochemistry & Molecular Biology Chemistry Polymer Science
Divisions:
Page Range: pp. 962-972
Journal or Publication Title: International Journal of Biological Macromolecules
Journal Index: ISI
Volume: 208
Identification Number: https://doi.org/10.1016/j.ijbiomac.2022.03.146
ISSN: 0141-8130
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4003

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