Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Changes in the expression of miR-103a and miR-21: a functional diagnosis of toxocariasis in rats

Thu Feb 2 18:12:01 2023

(2022) Changes in the expression of miR-103a and miR-21: a functional diagnosis of toxocariasis in rats. Journal of Medical Microbiology. p. 7. ISSN 0022-2615

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Abstract

Introduction. Toxocariasis is a zoonotic parasitic disease caused by migrating nematode worms. Toxocara species larvae, within tissues. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression at a post-transcriptional level. Hypothesis/Gap Statement. miRNA-based diagnostic biomarkers for toxocariasis are emerging, but there is limited information about the role of many miRNAs and a more detailed diagnostic evaluation of miRNA expression patterns is needed to understand their immunobiological function. Aim. We investigated the expression levels of circulating miRNA 21 and miRNA 103a as potential biomarkers for the prediction and diagnosis of toxocariasis in Wistar rats infected with Toxocara canis. Methodology. Thirty Wistar rats were inoculated orally with 2500 T. canis embryonated eggs via gavage. Serum samples were collected from infected animals and were tested against T. canis antigens for 60 days post-infection. The plasma samples were isolated for quantitative real-time PCR (qPCR) assays and qPCR was used to assess transcription levels of miRNA 21 and miRNA 103a. Results. The prevalence of anti-Toxocara IgG was detected in 7/30 (23.3) infected rats. Molecular analysis of miRNAs 21 and 103a showed that expression levels of miRNAs in both groups of Toxocara-positive and negative samples were the same without significant association. The ratio of housekeeping gene expression (U6) to gene expression of miRNAs 21 and 103a indicated the rate of change (1/1.38 approximate to 0.75 and 1/0.751 approximate to 1.3, respectively). Conclusion. Our study revealed that miRNAs 21 and 103a might play fundamental roles as biomarkers and diagnostic tools for toxocariasis. However, the changes in expression of these miRNAs were not adequate to be used as biomarkers in diagnosis.

Item Type: Article
Creators:
CreatorsEmail
Zibaei, M.UNSPECIFIED
Rokni, M. B.UNSPECIFIED
Sohrabi, N.UNSPECIFIED
Sepahvand, A.UNSPECIFIED
Raiesi, O.UNSPECIFIED
Getso, M. I.UNSPECIFIED
Alizadeh, G.UNSPECIFIED
Ibrahim, A.UNSPECIFIED
Yarahmadi, M.UNSPECIFIED
Raissi, V.UNSPECIFIED
Keywords: Toxocara canis expression miRNA 21 miRNA 103a quantitative real-time PCR ELISA canis infection contamination prevalence gerbils region risk rna Microbiology
Divisions:
Page Range: p. 7
Journal or Publication Title: Journal of Medical Microbiology
Journal Index: ISI
Volume: 71
Number: 5
Identification Number: https://doi.org/10.1099/jmm.0.001532
ISSN: 0022-2615
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3995

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