Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Neospora caninum SRS2 Protein: Essential Vaccination Targets and Biochemical Features for Next-Generation Vaccine Design

Sun Apr 21 00:07:21 2024

(2022) Neospora caninum SRS2 Protein: Essential Vaccination Targets and Biochemical Features for Next-Generation Vaccine Design. Biomed Research International. p. 13. ISSN 2314-6133

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Abstract

Vaccination is a standout preventive measure to combat neosporosis among cattle herds. The present in silico study was done to evaluate the physicochemical properties and potent immunogenic epitopes of N. caninum SRS2 protein as a possible vaccine candidate. Web-based tools were used to predict physicochemical properties, antigenicity, allergenicity, solubility, posttranslational modification (PTM) sites, transmembrane domains and signal peptide, and secondary and tertiary structures as well as intrinsically disordered regions, followed by identification and screening of potential linear and conformational B-cell epitopes and those peptides having affinity to bind mouse major histocompatibility complex (MHC) and cytotoxic T lymphocyte (CTL). The protein had 401 residues with a molecular weight of 42 kDa, representing aliphatic index of 69.35 (thermotolerant) and GRAVY score of -0.294 (hydrophilic). There were 53 PTM sites without a signal peptide in the sequence. Secondary structure comprised mostly by extended strand, followed by helices and coils. The Ramachandran plot of the refined model showed 90.2, 8.8, 0.5, and 0.5 residues in the favored, additional allowed, generously allowed, and disallowed regions, correspondingly. Additionally, various potential B-cell (linear and conformational), CTL, and MHC-binding epitopes were predicted for N. caninum SRS2. These epitopes could be further utilized in the multiepitope vaccine constructs directed against neosporosis.

Item Type: Article
Creators:
CreatorsEmail
Asghari, A.UNSPECIFIED
Kordi, B.UNSPECIFIED
Maleki, B.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Shams, M.UNSPECIFIED
Naserifar, R.UNSPECIFIED
Keywords: multi-epitope vaccine in-silico analysis b-cell epitopes toxoplasma-gondii immunogenic epitopes definitive hosts surface-antigen bioinformatics analysis recombinant ncsrs2 immune-response Biotechnology & Applied Microbiology Research & Experimental Medicine
Divisions:
Page Range: p. 13
Journal or Publication Title: Biomed Research International
Journal Index: ISI
Volume: 2022
Identification Number: https://doi.org/10.1155/2022/7070144
ISSN: 2314-6133
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3943

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