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Ilam University of Medical Sciences

Molecular characterization of Trichomonas infections in women of Ilam City, southwestern Iran

Thu Feb 2 17:44:43 2023

(2022) Molecular characterization of Trichomonas infections in women of Ilam City, southwestern Iran. Parasitology Research. pp. 1631-1638. ISSN 0932-0113

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Abstract

Trichomoniasis is a sexually transmitted infection (STI) caused by the flagellated protozoan Trichomonas vaginalis. Little information is available on the epidemiology and genetic diversity of T. vaginalis in Ilam City, southwestern Iran. A descriptive cross-sectional investigation was carried out between July 2017 and December 2018 on the suspected women patients referred to eight gynecology clinics of Ilam City for probable Trichomonas infection. They were undergone a set of clinical, parasitological, and molecular examinations. During clinical consultation, posterior vaginal fornix secretions and urine samples were gathered from the participants. For the reasons such as physical conditions and cultural and religious constraints, most of participating women, especially young girls due to their virginity, preferred to give urine samples instead of vaginal discharge. The presence of Trichomonas was diagnosed by microscopic examination and molecular detection using conventional PCR targeting ITS1-rDNA. A total of 1765 suspected individuals were examined clinically via vaginal secretions (495 specimens) and urine samples (1270 specimens). Of them, 21 (1.18) cases, including 13 vaginal secretions and 8 urine samples, were positive for Trichomonas infection by microscopy. Slightly more than half of the patients (11/21, 52.4) complained of vulvar itching, burning, and frequent urination. Cervical lesions, patchy erythema, and vaginal discharge were recorded in 28.6, 23.8, and 19 of the patients respectively. All patients with positive microscopic identification were confirmed by amplification of 450-bp fragment of ITS1-rDNA. Phylogenetic analysis revealed a high rate of genetic homogeneity in which all our isolates together with homologous sequences from China, Philippines, Austria, and USA were clustered within the same clade. A statistically significant relationship was recorded between the patients positive for trichomoniasis and the presence of chronic disease (e.g., diabetes, immune system deficiency).

Item Type: Article
Creators:
CreatorsEmail
Alikhani, M.UNSPECIFIED
Akhoundi, M.UNSPECIFIED
Sereno, D.UNSPECIFIED
Abdi, J.UNSPECIFIED
Naserifar, R.UNSPECIFIED
Mahmoudi, M. R.UNSPECIFIED
Mirzaei, A.UNSPECIFIED
Keywords: Trichomonas vaginalis Molecular characterization Genetic diversity ITS1-rDNA Ilam vaginalis infection genetic diversity prevalence diagnosis epidemiology polymorphism pcr Parasitology
Divisions:
Page Range: pp. 1631-1638
Journal or Publication Title: Parasitology Research
Journal Index: ISI
Volume: 121
Number: 6
Identification Number: https://doi.org/10.1007/s00436-022-07492-w
ISSN: 0932-0113
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3935

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