Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Determination of B and T Cell Epitopes in Neospora caninum Immune Mapped Protein-1 (IMP-1): Implications in Vaccine Design against Neosporosis

Sat Jul 13 04:19:08 2024

(2022) Determination of B and T Cell Epitopes in Neospora caninum Immune Mapped Protein-1 (IMP-1): Implications in Vaccine Design against Neosporosis. Biomed Research International. p. 12. ISSN 2314-6133

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Prevention of neosporosis is advantageous for cattle health and productivity. Previously, several vaccine candidates were nominated for vaccination against Neospora caninum. This study was premised on in silico evaluation of N. caninum IMP-1 in order to determine its physicochemical features and immunogenic epitopes. We employed a wide array of network-based tools for the prediction of antigenicity, allergenicity, solubility, posttranslational modification (PTM) sites, physicochemical properties, transmembrane domains and signal peptide, secondary and tertiary structures, and intrinsically disordered regions. Also, prediction and screening of potential continuous B cell peptides and those epitopes having stringent affinity to couple with mouse major histocompatibility complex (MHC) and cytotoxic T lymphocyte (CTL) receptors were accomplished. The protein had 393 residues with a molecular weight of 42.71 kDa, representing aliphatic index of 85.83 (thermotolerant) and GRAVY score of -0.447 (hydrophilic). There were 47 PTM sites without a signal peptide in the sequence. Secondary structure comprised mostly of extended strand and helices, followed by coils. The Ramachandran plot of the refined model showed 90.1, 9.9, 0.0, and 0.0 residues in the favored, additional allowed, generously allowed, and disallowed regions, correspondingly. Additionally, various potential B cell (linear and conformational), CTL, and MHC binding epitopes were predicted for N. caninum IMP-1. The findings of the present study could be further directed for next-generation vaccine design against neosporosis.

Item Type: Article
Creators:
CreatorsEmail
Nazari, N.UNSPECIFIED
Kordi, B.UNSPECIFIED
Maleki, B.UNSPECIFIED
Shams, M.UNSPECIFIED
Azizi, E.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Naserifar, R.UNSPECIFIED
Keywords: definitive hosts bioinformatics analysis balb/c mice infection identification candidate antigens abortion cattle Biotechnology & Applied Microbiology Research & Experimental Medicine
Divisions:
Page Range: p. 12
Journal or Publication Title: Biomed Research International
Journal Index: ISI
Volume: 2022
Identification Number: https://doi.org/10.1155/2022/2508050
ISSN: 2314-6133
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3934

Actions (login required)

View Item View Item