Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Genotyping of Listeria monocytogenes isolates by high-resolution melting curve (HRM) analysis of tandem repeat locus

Thu Feb 2 17:47:06 2023

(2022) Genotyping of Listeria monocytogenes isolates by high-resolution melting curve (HRM) analysis of tandem repeat locus. Brazilian Journal of Infectious Diseases. p. 7. ISSN 1413-8670

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Abstract

Listeria monocytogenes is responsible for causing listeriosis, a type of food poisoning with high mortality. This bacterium is mainly transmitted to humans through the consumption of contaminated foods. Detection of L. monocytogenes through molecular methods is crucial for food safety and clinical diagnosis. Present techniques are characterized by low discrimination power and high cost, as well as being time-consuming and taking several days to give the final result. In our study, MLVA-HRM (Multiple-Locus Variable-number tandem repeats Analysis - High-Resolution Melting) was investigated as an alternative method for a fast and precise method for the genotyping of L. monocytogenes isolates. Forty-eight isolates of L. monocytogenes obtained from the microbial bank of Department of Microbiology, Iran University of Medical Sciences, were typed by MLVA-HRM analysis using five Variable Numbers of Tandem Repeat (VNTR) loci. A total of 43 different types were obtained. This research demonstrated the usefulness of the MLVA-HRMA method and its ability to discriminate L. monocytogenes isolates. Since this method is easier and more efficient than existing methods, it can be widely used in food processing plants and diagnostic laboratories as a fast and accurate method.(c) 2022 Published by Elsevier Espana, S.L.U. on behalf of Sociedade Brasileira de Infectologia. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Item Type: Article
Creators:
CreatorsEmail
Narimisa, N.UNSPECIFIED
Amraei, F.UNSPECIFIED
Sholeh, M.UNSPECIFIED
Mirkalantari, S.UNSPECIFIED
Razavi, S.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Lotfollahi, L.UNSPECIFIED
Jazi, F. M.UNSPECIFIED
Keywords: Listeria monocytogenes MLVA-HRMA VNTR locus Molecular typing capillary-electrophoresis strains contamination mlva Infectious Diseases
Divisions:
Page Range: p. 7
Journal or Publication Title: Brazilian Journal of Infectious Diseases
Journal Index: ISI
Volume: 26
Number: 2
Identification Number: https://doi.org/10.1016/j.bjid.2022.1023481413-8670
ISSN: 1413-8670
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3931

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