Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

relBE toxin-antitoxin system as a reliable anti-biofilm target in Pseudomonas aeruginosa

Mon Jun 24 04:23:12 2024

(2022) relBE toxin-antitoxin system as a reliable anti-biofilm target in Pseudomonas aeruginosa. Journal of Applied Microbiology. pp. 683-695. ISSN 1364-5072

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Aims The ability of the pathogenic bacterium Pseudomonas aeruginosa to produce biofilms has made it more difficult to treat its infections with current antibiotics. Several genes are involved in biofilm production, and toxin-antitoxin (TA) loci have been reported to be responsible for the regulation of biofilm-associated genes. This study was aimed at evaluating various TA loci in P. aeruginosa to find a reliable target in order to disrupt biofilm formation. Methods and Results Thirty clinical isolates of P. aeruginosa were assessed for biofilm production as well as the presence of various TA loci in their genomes. The relBETA locus was present in all 30 P. aeruginosa isolates but its expression was not detectable in isolates that did not show biofilm production. Quantitative real-time -PCR (q-PCR) also demonstrated that the expression of relBE was higher in isolates with stronger biofilm-producing capability. Knocking out the relBE locus in one biofilm-producing P. aeruginosa isolate led to the cessation of biofilm-producing capacity in that isolate and eliminated the expression of ndvB, which is among the genes involved in biofilm production. Conclusions These results inferred the involvement of relBE TA locus in the regulation of biofilm production in P. aeruginosa and indicated the possibility of relBE as an anti-biofilm target for this pathogen.

Item Type: Article
Sadeghifard, N.UNSPECIFIED
Keywords: biofilm production Pseudomonas aeruginosa relBE target discovery toxin-antitoxin systems toxin/antitoxin system risk-factors tolerance agents Biotechnology & Applied Microbiology Microbiology
Page Range: pp. 683-695
Journal or Publication Title: Journal of Applied Microbiology
Journal Index: ISI
Volume: 133
Number: 2
Identification Number:
ISSN: 1364-5072
Depositing User: مهندس مهدی شریفی

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