Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Effect of Chlorhexidine (CHX) and Hydrogen Peroxide (H2O2) on the Biofilm Formation of Enterococcus faecalis

Sun Jun 16 06:49:55 2024

(2022) Effect of Chlorhexidine (CHX) and Hydrogen Peroxide (H2O2) on the Biofilm Formation of Enterococcus faecalis. Clinical Laboratory. pp. 673-680. ISSN 1433-6510

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Background: Biofilm makes bacteria resistant to antimicrobial agents and facilitates the transmission of infectious diseases in hospitals. Disinfectant compounds are frequently used to control surface contamination. This study was designed to investigate the effect of chlorhexidine (CHX) and hydrogen peroxide (H2O2) on biofilm formation of Enterococcus faecalis. Methods: This study was performed on 40 E. faecalis clinical isolates. After the determination of MIC, the effect of different concentrations of CHX and H2O2 on the biofilm formation was evaluated. Also, the relative expression level of the studied biofilm genes, following exposure to sublethal concentration of CHX and H2O2, was assessed using quantitative reverse transcription PCR (qRT-PCR). Results: The frequency of the asa1, efaA, epaI, and esp biofilm genes were 80, 92.5, 100, and 75, respectively. Various concentrations of CHX increased the biofilm mass in E. faecalis. Also, the combination of CHX and H2O2 at sub-minimal inhibitory concentrations, significantly elevated the expression of asa1, epaI, and esp genes. Conclusions: The results of this study showed that the improper use of disinfectants can increase the ability of biofilm formation in E. faecalis and may cause selective pressure leading to the emergence of biocide-resistant micro-organisms.

Item Type: Article
Sadeghifard, N.UNSPECIFIED
Azizi-Jalilian, F.UNSPECIFIED
Keywords: biofilm formation enterococci CHX and H2O2 qRT-PCR vancomycin-resistant enterococci surveillance infections Medical Laboratory Technology
Page Range: pp. 673-680
Journal or Publication Title: Clinical Laboratory
Journal Index: ISI
Volume: 68
Number: 4
Identification Number:
ISSN: 1433-6510
Depositing User: مهندس مهدی شریفی

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