Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Inhibitory Effects of Carvacrol on Biofilm Formation and Expression of Biofilm Related Genes in Clinical Isolates of Enterococcus faecalis

Thu Feb 2 18:58:58 2023

(2022) Inhibitory Effects of Carvacrol on Biofilm Formation and Expression of Biofilm Related Genes in Clinical Isolates of Enterococcus faecalis. Clinical Laboratory. pp. 1205-1211. ISSN 1433-6510

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Abstract

Background: Nowadays, novel antimicrobial strategies are being developed which focus on debilitating, rather than killing the microorganisms. In this regard, anti-biofilm therapy is one of the important ways to combat bacterial infections. Therefore, the aim of the current study was to evaluate the anti-biofilm activity of Carvacrol against E. faecalis by means of its effects on biofilm formation as well as on the gene expression levels of the two biofilm related genes, Epa and Esp. Methods: A total of 40 clinical strains of E. faecalis were collected from three hospitals in Tehran, Iran during 2020. These isolates were confirmed by biochemical and genotypic methods. Antibacterial and anti-biofilm activity of Carvacrol essence were determined according the standard protocol. Finally, expression level of the biofilm related genes (Epa and Esp) were evaluated before and after the treatment with Carvacrol. Results: A total of 14 isolates were considered as strong biofilm producers and were used for analysis. Carvacrol essence showed the best antibacterial activity at 2,500 mu g/mL concentration against all the isolates, the biofilm formation capacity was decreased by Carvacrol essence, and it was statistically significant (p < 0.05). Expression levels of the Esp gene were decreased in 5 isolates while increased in 3 isolates following the Carvacrol treatment. Expression levels of the EpaI gene was significantly decreased (p < 0.05) in 4 isolates following the Carvacrol treatment. Conclusions: In conclusion, the results presented in this study suggest that carvacrol extract exhibits significant antimicrobial and anti-biofilm properties against E. faecalis, even against vancomycin resistant isolates.

Item Type: Article
Creators:
CreatorsEmail
Yarkarami, F.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Pakzad, R.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Asadollahi, P.UNSPECIFIED
Hematian, A.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Keywords: carvacrol biofilm anti-biofilm E. faecalis surface protein resistance infections virulence esp Medical Laboratory Technology
Divisions:
Page Range: pp. 1205-1211
Journal or Publication Title: Clinical Laboratory
Journal Index: ISI
Volume: 68
Number: 6
Identification Number: https://doi.org/10.7754/Clin.Lab.2021.210853
ISSN: 1433-6510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3907

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