Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Novel Antimicrobial Target in Acinetobacter Baumannii

Wed Oct 4 02:50:16 2023

(2022) Novel Antimicrobial Target in Acinetobacter Baumannii. Clinical Laboratory. pp. 2305-2308. ISSN 1433-6510

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Abstract

Background: Resistance to multiple drugs is one of the biggest challenges in managing infectious diseases. Acinetobacter baumannii is considered a nosocomial infection. According to the multiple roles of the toxin-antitoxin system, this system can be considered an antimicrobial target in the presence of bacteria. With the impact on bacterial toxin, it can be used as a new antibacterial target. The purpose of this study was to determine the mazEF genes as a potent antimicrobial target in A. baumannii clinical isolates. Methods: The functionality of mazEF genes was evaluated by qPCR in fifteen A. baumannii clinical isolates. Then, the mazE locus was targeted by peptide nucleic acid (PNA). Results: The results showed a significant difference in the mean number of copies of mazF gene in normal and stress conditions. Also, we found that at a concentration of 15 mu M of PNA the bacteria were killed and confirmed by culture on LB agar. Conclusions: This research is the first step in introducing mazEF TA loci as a sensitive target in A. baumannii. However, more studies are needed to test the effectiveness in vivo. In addition, the occurrence and potential for activation of the TA system, mazEF in other pathogenic bacteria should be further investigated.

Item Type: Article
Creators:
CreatorsEmail
Norouzi, M.UNSPECIFIED
Mahmoudi, M.UNSPECIFIED
Maleki, A.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Keywords: Acinetobacter baumannii toxin-antitoxin system pep-tide nucleic acid toxin-antitoxin system Medical Laboratory Technology
Divisions:
Page Range: pp. 2305-2308
Journal or Publication Title: Clinical Laboratory
Journal Index: ISI
Volume: 68
Number: 5
Identification Number: https://doi.org/10.7754/Clin.Lab.2021.210728
ISSN: 1433-6510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3900

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