Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

In Vitro and In Silico Investigation of some Type II TA Genes in H. Pylori

Thu Feb 2 17:56:18 2023

(2022) In Vitro and In Silico Investigation of some Type II TA Genes in H. Pylori. Clinical Laboratory. pp. 1596-1604. ISSN 1433-6510

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Abstract

Background: In addition to antibiotic resistance, the entry of Helicobacter pylori into the persistence phase leads to recurrent and chronic infections, as well as the development of antibiotic resistance in persister cells. Methods: In this study, after genetic confirmation of H. pylori in 20 biopsy specimens, the prevalence of the type II TA systems mazEF, relEB, yafQ/dinJ was investigated. Also, the most common system observed in the study in terms of structure, evolution, and molecular interaction was evaluated by bioinformatics tools. Results: The results of the PCR test on 20 biopsy samples were positive for ureA and glmM genes. Moreover, yafQ/ dinJ was the only module positive in half of the samples (10 samples) in the PCR technique. The toxin residues and their interactions with the cognate antitoxin residues are revealed by docking analysis results. Furthermore, the multiple sequence alignment (MSA) of the YafQ toxin showed that this toxin has a low polymorphism among H. pylori species. The evolutionary study showed that the yafQ toxin had the highest sequence similarity among the bacteria Helicobacter cetorm (60 similarity) and Muricauda olearia (57.35 similarity). Conclusions: Collectively, the data of the present study indicate that the YafQ/DinJ is the dominant type II TA system and has the highest frequency among the studied systems in H. pylori, and further studies are required to elucidate its exact role in this bacterium.

Item Type: Article
Creators:
CreatorsEmail
Hoseini, S.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Maleki, A.UNSPECIFIED
Asadollahi, P.UNSPECIFIED
Badakhsh, B.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Keywords: H pylori toxin-antitoxin YafQ DinJ system structure alignment docking new target protein prediction server Medical Laboratory Technology
Divisions:
Page Range: pp. 1596-1604
Journal or Publication Title: Clinical Laboratory
Journal Index: ISI
Volume: 68
Number: 8
Identification Number: https://doi.org/10.7754/Clin.Lab.2021.211002
ISSN: 1433-6510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3897

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