Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Construction of a multi-epitope protein for human Toxocara canis detection: Immunoinformatics approach multi-epitope construct for T. canis serodiagnosis

Sun Nov 27 23:16:39 2022

(2021) Construction of a multi-epitope protein for human Toxocara canis detection: Immunoinformatics approach multi-epitope construct for T. canis serodiagnosis. Informatics in Medicine Unlocked. ISSN 23529148 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Toxocariasis is a global public health concern, particularly in children. Serological diagnosis of the infection is routinely applied to detect specific anti-Toxocara antibodies. Current in silico study was contrived to design a multi-epitope protein using a wide array of immunoinformatics web servers for improved serodiagnosis. Top, high-ranked B-cell epitopes as well as IL-4 and IL-10 inducing epitopes were predicted, screened and selected to construct the chimeric protein. Then, prediction of antigenicity, solubility and physico-chemical features, secondary and tertiary structures, 3D model refinement and validations was done using various web servers. Finally, conformational B-cell epitopes were determined for possible protein-antibody interactions. Moreover, the final construct was subjected to disulfide engineering, codon adaptation, in silico cloning as well as molecular docking with human TLR-2 molecule. In conclusion, this multi-epitope construct needs to be evaluated experimentally, in order to be used in commercial serodiagnostic kits. © 2021

Item Type: Article
Creators:
CreatorsEmail
Shams, M.UNSPECIFIED
Nourmohammadi, H.UNSPECIFIED
Asghari, A.UNSPECIFIED
Basati, G.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Naserifar, R.UNSPECIFIED
Irannejad, H.UNSPECIFIED
Keywords: Immunoinformatics Multi-epitope Serodiagnosis Synthetic protein Toxocara canis
Divisions:
Journal or Publication Title: Informatics in Medicine Unlocked
Journal Index: Scopus
Volume: 26
Identification Number: https://doi.org/10.1016/j.imu.2021.100732
ISSN: 23529148 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3892

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