Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Serum level and tumor tissue expression of Ribonucleotide-diphosphate Reductase subunit M2 B: a potential biomarker for colorectal cancer

Sun Jul 14 17:51:23 2024

(UNSPECIFIED) Serum level and tumor tissue expression of Ribonucleotide-diphosphate Reductase subunit M2 B: a potential biomarker for colorectal cancer. Molecular Biology Reports. p. 7. ISSN 0301-4851

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Abstract

Background This study explored the applicability of serum level and tissue expression of Ribonucleotide-diphosphate Reductase subunit M2 B (RRM2B) as reliable biomarkers for colorectal cancer (CRC) progression and metastasis. Methods and results The present descriptive-analytic cohort study was conducted on 50 newly diagnosed CRC patients (stage II, III) and 50 healthy individuals. The new cases had not received any therapeutic intervention and underwent surgery immediately after the initial diagnosis. Tumorous tissues and marginal healthy tissues (as control) were excised to determine the mRNA tissue expression of RRM2B by Real-Time PCR. Serum RRM2B protein was measured using an ELISA method once in the control group. In the patients, serum RRM2B protein was evaluated before, 1 and 3 months after surgery. The tumor metastasis node (TMN) classification system and liver metastasis were evaluated in CRC patients. The results showed significantly lower RRM2B serum levels in 1 and 3 months after surgery compared with the pre-surgery condition (P = 0.014, P < 0.001 respectively). The mean RRM2B gene expression was 51 lower in tumor tissue than its adjacent normal tissue (P < 0.001). No significant relationship was found between serum level of RRM2B and tumor staging and metastasis in patients before surgery (P = 0.373, P = 0.189), 1 month after surgery (P = 0.960, P = 0.088), and 3 months after surgery (P = 0.407, P = 0.724). RRM2B expression in tumor tissue is not associated with tumor staging and metastasis (P = 0.254, P = 0.721). Conclusion These data suggest measuring serum protein level of RRM2B could have a role in CRC progression, although this study should be considered preliminary due to small sample size and short follow-up duration.

Item Type: Article
Creators:
CreatorsEmail
Mobarra, N.UNSPECIFIED
Gholamalizadeh, H.UNSPECIFIED
Abdulhussein, K. A.UNSPECIFIED
Raji, S.UNSPECIFIED
Asl, F. T.UNSPECIFIED
Mirvahabi, M. S.UNSPECIFIED
Rafiee, M.UNSPECIFIED
Pakzad, R.UNSPECIFIED
Keywords: Colorectal cancer RRM2B Liver metastasis Prognosis Biomarker p53r2 gene metastasis survival overexpression proliferation progression carcinoma pathway growth Biochemistry & Molecular Biology
Divisions:
Page Range: p. 7
Journal or Publication Title: Molecular Biology Reports
Journal Index: ISI
Identification Number: https://doi.org/10.1007/s11033-022-07205-7
ISSN: 0301-4851
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3841

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