Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Immunoinformatics Insights into the Internalin A and B Proteins to Design a Multi-Epitope Subunit Vaccine for L. monocytogenes

Thu Aug 11 16:26:08 2022

(2022) Immunoinformatics Insights into the Internalin A and B Proteins to Design a Multi-Epitope Subunit Vaccine for L. monocytogenes. International Journal of Peptide Research and Therapeutics. p. 10. ISSN 1573-3149

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Abstract

As an important foodborne pathogen, Listeria monocytogenes is associated with high hospitalization and case-fatality rates. Outbreaks due to foods contaminated with this pathogen continue to occur globally. A suitable vaccine can protect susceptible populations against this infection. In this study, two essential surface proteins (internalins A and B) of L. monocytogenes which are used for the entry of bacteria into different host cells were selected as candidates for the design of a multi-epitope protein vaccine by powerful immunoinformatics servers and soft wares. Epitopes were selected based on scores of antigenic properties, cytokine induction potential and allergenicity. To construct the multi-epitope vaccine, the selected cytotoxic T lymphocytes epitopes were linked together by an AAY linker, whereas GPGPG linkers were used to link the helper T lymphocytes epitopes. Physiochemical characterization, and the secondary and tertiary structures of the protein vaccine were determined. After refinement, validation and energy minimization, the immune simulation was conducted by the C-ImmSim server. The obtained results showed that the designed vaccine is stable and could effectively trigger the immune response. Overall, the results of this study demonstrated that the designed vaccine is specific, antigenic, non-allergenic, and can effectively prevent L. monocytogenes infections. Further clinical trials are required to check the efficacy of this vaccine.

Item Type: Article
Creators:
CreatorsEmail
Asadollahi, P.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Kaviar, V. H.UNSPECIFIED
Fattahi, R.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Keywords: Listeria monocytogenes Vaccine Internalin Immunoinformatics Epitope listeria-monocytogenes web Biochemistry & Molecular Biology
Divisions:
Page Range: p. 10
Journal or Publication Title: International Journal of Peptide Research and Therapeutics
Journal Index: ISI
Volume: 28
Number: 1
Identification Number: https://doi.org/10.1007/s10989-021-10359-w
ISSN: 1573-3149
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3814

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