Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Hybrid poly-l-lactic acid/poly(ε-caprolactone) nanofibrous scaffold can improve biochemical and molecular markers of human induced pluripotent stem cell-derived hepatocyte-like cells

Wed Jun 19 07:48:33 2024

(2019) Hybrid poly-l-lactic acid/poly(ε-caprolactone) nanofibrous scaffold can improve biochemical and molecular markers of human induced pluripotent stem cell-derived hepatocyte-like cells. Journal of Cellular Physiology. pp. 11247-11255. ISSN 00219541 (ISSN)

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A suitable alternative strategy for liver transplantation is the use of nanofibrous scaffolds together with stem cells. In this study, a random hybrid of poly-l-lactic acid (PLLA) and poly(ε-caprolactone) (PCL) was used as a three-dimensional (3D) culture for differentiation of hepatocyte-like cells and compared with routine culture (two-dimensional 2D). The expression of the endodermal marker, forkhead box A2 (FOXA2), was assessed on Day 3 and the hepatic markers; albumin (ALB), α-1 antitrypsin (AAT), and cytokeratin-18 (CK-18) were evaluated on Day 18 using quantitative polymerase chain reaction qPCR. As well as, ALB, α-fetoprotein (AFP), and low-density lipoprotein (LDL) uptake were evaluated using immunocytochemistry; moreover, periodic acid-Schiff and Oil Red were done by cell staining. In addition, AFP and urea production were evaluated by chemiluminescence and colorimetric assays. Light and scanning electron microscopy (SEM) showed changes in the cells in 2D and 3D models. The gene expression of hepatic markers was significantly higher in the 3D cultures. In addition, immunocytochemistry and cell staining showed that ALB, AFP, LDL-uptake, periodic acid-Schiff, and Oil Red were expressed in both cells derived on 2D and 3D. Furthermore, the evaluation of AFP and urea secretion was significantly different between 2D and 3D strategies. These findings suggest that functionally cells cultured on a PLLA/PCL scaffold may be suitable for cell therapy and regenerative medicine. © 2018 Wiley Periodicals, Inc.

Item Type: Article
Ghayour-Mobarhan, M.UNSPECIFIED
Keywords: hepatocyte human induced pluripotent stem cells nanofiber PLLA/PCL scaffold albumin alpha 1 antitrypsin alpha fetoprotein cytokeratin 18 hepatocyte nuclear factor 3beta lipoprotein molecular marker molecular scaffold polycaprolactone polylactic acid albuminoid FOXA2 protein, human lactic acid low density lipoprotein polyester animal cell Article biochemical analysis cell culture cell differentiation chemoluminescence colorimetry controlled study embryo endoderm gene expression human human cell immunocytochemistry immunofluorescence immunohistochemistry in vitro study induced pluripotent stem cell liver cell microscopy mouse nanofabrication nonhuman polymerase chain reaction priority journal protein expression quantitative analysis scanning electron microscopy urea cycle animal biological therapy chemistry cytology liver transplantation metabolism procedures tissue engineering tissue scaffold Albumins alpha-Fetoproteins Animals Cell- and Tissue-Based Therapy Cells, Cultured Hepatocyte Nuclear Factor 3-beta Hepatocytes Humans Induced Pluripotent Stem Cells Keratin-18 Lipoproteins, LDL Mice Nanofibers Polyesters Tissue Scaffolds
Page Range: pp. 11247-11255
Journal or Publication Title: Journal of Cellular Physiology
Journal Index: Scopus
Volume: 234
Number: 7
Identification Number:
ISSN: 00219541 (ISSN)
Depositing User: مهندس مهدی شریفی

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