Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Identification of metallo-β-lactamases and AmpC production among Escherichia coli strains isolated from hemodialysis patients with urinary tract infection

Tue Aug 16 11:07:59 2022

(2021) Identification of metallo-β-lactamases and AmpC production among Escherichia coli strains isolated from hemodialysis patients with urinary tract infection. Molecular Biology Reports. pp. 7883-7892. ISSN 03014851 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: This study aimed to identify metallo-β-lactamases (MBLs) and AmpC β-lactamases-producing Escherichia coli isolates obtained from hemodialysis (HD) patients with urinary tract infections (UTI). Methods and results: A total of 257 HD patients with UTI were included in this study, from which 47 E. coli isolates were collected. Antibiotic susceptibility was tested by disc diffusion method. MBLs and AmpC production were phenotypically detected by imipenem-ethylenediaminetetracetate and cefoxitin/boronic acid assays, respectively. The presence of MBLs and AmpC genes was examined by polymerase chain reaction (PCR). Fosfomycin and ampicillin were the most and the least effective antibiotics against E. coli isolates, respectively. Moreover, 61.7 (29/47) of E. coli isolates were multidrug-resistant with seven different antibiotypes. Antibiotype V (AMP–CIP–IMP–MEM–CPD–CRO–CTX–GEN–LEV–SXT–TOB) was the most prevalent profile. Besides, 24 (51.1) isolates were simultaneously resistant to imipenem and meropenem. Phenotypic assay showed MBL production in 16 (66.7) of the 24 carbapenem-resistant E. coli isolates. The distribution of MBL genes in carbapenem-resistant E. coli was as follows: blaIMP 18 (72), blaVIM 7 (28), and blaNDM 1 (4). AmpC was detected in 61.7 (29/47) of the isolates using the phenotypic method. The presence of AmpC genes was confirmed by PCR in only 26 of 29 (86.7) AmpC producers. The frequencies of blaDHA-1, blaACC, and blaCMY-2 were 6 (20.7), 11 (37.9), and 21 (72.4), respectively. Conclusions: The emergence of MBL and AmpC coproducing E. coli isolates calls for an urgent surveillance program for timely diagnosis and screening of these genes in our healthcare systems. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.

Item Type: Article
Creators:
CreatorsEmail
Bahramian, A.UNSPECIFIED
Khoshnood, S.UNSPECIFIED
Hashemi, N.UNSPECIFIED
Moradi, M.UNSPECIFIED
Karimi-Yazdi, M.UNSPECIFIED
Jalallou, N.UNSPECIFIED
Saki, M.UNSPECIFIED
Keywords: AmpC Escherichia coli Hemodialysis patients Metallo-β-lactamases Urinary tract infections amikacin ampicillin beta lactamase AmpC boronic acid derivative cefotaxime cefoxitin ceftazidime ceftriaxone cotrimoxazole fosfomycin gentamicin imipenem levofloxacin meropenem metallo beta lactamase nitrofurantoin piperacillin plus tazobactam tobramycin adolescent adult aged AmpC gene antibiotic sensitivity Article bacterial strain bacterium isolate carbapenem resistant Escherichia coli child cross-sectional study disk diffusion female hemodialysis human major clinical study male MBL gene multidrug resistance polymerase chain reaction protein analysis protein synthesis urinary tract infection
Divisions:
Page Range: pp. 7883-7892
Journal or Publication Title: Molecular Biology Reports
Journal Index: Scopus
Volume: 48
Number: 12
Identification Number: https://doi.org/10.1007/s11033-021-06814-y
ISSN: 03014851 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3775

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