Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism

Tue Aug 16 11:05:40 2022

(2021) Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism. BioMed research international. p. 1315618. ISSN 2314-6141 (Electronic)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/34692826

Abstract

Toxoplasmosis is a global threat with significant zoonotic concern. The present in silico study was aimed at determination of bioinformatics features and immunogenic epitopes of a tyrosine-rich oocyst wall protein (TrOWP) of Toxoplasma gondii. After retrieving the amino acid sequence from UniProt database, several parameters were predicted including antigenicity, allergenicity, solubility and physico-chemical features, signal peptide, transmembrane domain, and posttranslational modifications. Following secondary and tertiary structure prediction, the 3D model was refined, and immunogenic epitopes were forecasted. It was a 25.57 kDa hydrophilic molecule with 236 residues, a signal peptide, and significant antigenicity scores. Moreover, several linear and conformational B-cell epitopes were present. Also, potential mouse and human cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes were predicted in the sequence. The findings of the present in silico study are promising as they render beneficial characteristics of TrOWP to be included in future vaccination experiments.

Item Type: Article
Creators:
CreatorsEmail
Asghari, A.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Nemati, T.UNSPECIFIED
Fatollahzadeh, M.UNSPECIFIED
Shams, M.UNSPECIFIED
Naserifar, R.UNSPECIFIED
Kordi, B.UNSPECIFIED
Divisions:
Page Range: p. 1315618
Journal or Publication Title: BioMed research international
Journal Index: Pubmed
Volume: 2021
Identification Number: https://doi.org/10.1155/2021/1315618
ISSN: 2314-6141 (Electronic)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3764

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