Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Genotyping of Fasciola hepatica Isolated from Domestic Animals in the West of Iran

Mon Jul 22 15:03:37 2024

(2019) Genotyping of Fasciola hepatica Isolated from Domestic Animals in the West of Iran. Infectious disorders drug targets. pp. 409-413. ISSN 2212-3989 (Electronic) 1871-5265 (Linking)

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OBJECTIVES: The most common parasites responsible for fascioliasis are F. hepatica and F. gigantica. Fascioliasis is an important problem in terms of health and economics. The western part of Iran is one of the major hubs of animal husbandry and the prevalence of fascioliasis is high. The aim of the current study was to determine the Fasciola spp. present in the western part of Iran. In the present study, 45 samples were collected from slaughterhouses in three provinces in the western part of Iran, including Ilam, Lorestan and Kermanshah. METHODS: The flukes were detected using morphological methods and the DNA of all samples was extracted. The ribosomal internal transcribed spacer (ITS1) was identified by PCR and PCR-RFLP techniques using the Rsa1 restriction enzyme. Then, 15 of the samples were sequenced. RESULTS: Based on their ITS1 sequence, all samples showed 700 bp bands. The results of sequencing showed a similarity of 99 to 100 across samples. Using the Rsa1 restriction enzyme, all samples produced three distinct bands (60 bp, 100 bp, and 360 bp). These results demonstrated that the most common fasciola in the western part of Iran in sheep and cattle is F. hepatica. CONCLUSION: The results of the present study showed that only the species F. hepatica is parasitizing livestock in the western part of Iran. Further studies using new molecular markers for more accurate identification of fasciola-causing species will be useful in the control and prevention of fascioliasis.

Item Type: Article
Keywords: Animals Cattle/*parasitology DNA, Intergenic/genetics Fasciola hepatica/*genetics/isolation & purification Fascioliasis/epidemiology/*veterinary *Genotype Genotyping Techniques Iran/epidemiology Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Sheep, Domestic/*parasitology F. gigantica Fasciola hepatica Its1 Pcr-rflp fascioliasis genotyping.
Page Range: pp. 409-413
Journal or Publication Title: Infectious disorders drug targets
Journal Index: Pubmed
Volume: 19
Number: 4
Identification Number:
ISSN: 2212-3989 (Electronic) 1871-5265 (Linking)
Depositing User: مهندس مهدی شریفی

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