Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Sequential monitoring of TIM-3 mRNA expression in blood and urine samples of renal transplant recipients

Sun Sep 25 17:06:06 2022

(2019) Sequential monitoring of TIM-3 mRNA expression in blood and urine samples of renal transplant recipients. Transplant Immunology. pp. 9-16. ISSN 0966-3274

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Abstract

Background: T cell immunoglobulin and mucin domain 3 (TIM-3), as a co-inhibitory receptor expressed on Th1, Th17, CD8T, FoxP3 + Treg and innate immune cells, plays an important role in suppression of T cell-mediated immune responses, tolerance induction and T cell exhaustion. In this study, we evaluated sequential alterations of TIM-3 mRNA expression level in blood and urine samples of renal transplant recipients to predict approaching clinical episodes. Methods: A total of 52 adult renal transplant recipients (31 male and 21 female) were enrolled in this study. All the patients received kidney transplant from living unrelated donors. TIM-3 mRNA expression in peripheral blood mononuclear cells (PBMCs) and urinary cells were quantified using Real Time TaqMan polymerase chain reaction (PCR) at 4 different time points (pre-transplantation, 2, 90 and 180 days post-transplantation). Result: TIM-3 mRNA expression level on days 2, 90 and 180 after transplantation was significantly higher in blood and urine samples of patients with graft dysfunction (GD) compared with patients with well-functioning graft (WFG). Our results also showed a high correlation between blood and urinary level of TIM-3 mRNA expression. The data from Receiver Operating Characteristic (ROC) Curve Analysis showed that blood and urinary TIM-3 mRNA expression level at month 3 and 6 could discriminate graft dysfunction (GD) from well-functioning graft (WFG) with high specificity and sensitivity. Conclusion: Our data suggested that serial monitoring of TIM-3 mRNA level in the blood and urine samples of renal transplant recipients could be a useful non-invasive biomarker for prediction and diagnosis of allograft dysfunction.

Item Type: Article
Creators:
CreatorsEmail
Shahbaz, S. K.UNSPECIFIED
Barabadi, M.UNSPECIFIED
Ahmadpour, P.UNSPECIFIED
Pourrezagholi, F.UNSPECIFIED
Nafar, M.UNSPECIFIED
Foroughi, F.UNSPECIFIED
Hosseinzadeh, M.UNSPECIFIED
Ghorbanpour, M.UNSPECIFIED
Yekaninejad, M. S.UNSPECIFIED
Amirzargar, A.UNSPECIFIED
Keywords: TIM-3 Renal transplantation Graft dysfunction t-cells allograft rejection gene-expression granzyme-b kidney galectin-9 autoimmune induction diagnosis inflammation Immunology Transplantation
Divisions:
Page Range: pp. 9-16
Journal or Publication Title: Transplant Immunology
Journal Index: ISI
Volume: 54
Identification Number: https://doi.org/10.1016/j.trim.2018.10.007
ISSN: 0966-3274
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3742

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