Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Identification of metallo-beta-lactamases and AmpC production among Escherichia coli strains isolated from hemodialysis patients with urinary tract infection

Fri Aug 19 02:34:23 2022

(2021) Identification of metallo-beta-lactamases and AmpC production among Escherichia coli strains isolated from hemodialysis patients with urinary tract infection. Molecular Biology Reports. pp. 7883-7892. ISSN 0301-4851

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Abstract

Background This study aimed to identify metallo-beta-lactamases (MBLs) and AmpC beta-lactamases-producing Escherichia coli isolates obtained from hemodialysis (HD) patients with urinary tract infections (UTI). Methods and results A total of 257 HD patients with UTI were included in this study, from which 47 E. coli isolates were collected. Antibiotic susceptibility was tested by disc diffusion method. MBLs and AmpC production were phenotypically detected by imipenem-ethylenediaminetetracetate and cefoxitin/boronic acid assays, respectively. The presence of MBLs and AmpC genes was examined by polymerase chain reaction (PCR). Fosfomycin and ampicillin were the most and the least effective antibiotics against E. coli isolates, respectively. Moreover, 61.7 (29/47) of E. coli isolates were multidrug-resistant with seven different antibiotypes. Antibiotype V (AMP-CIP-IMP-MEM-CPD-CRO-CTX-GEN-LEV-SXT-TOB) was the most prevalent profile. Besides, 24 (51.1) isolates were simultaneously resistant to imipenem and meropenem. Phenotypic assay showed MBL production in 16 (66.7) of the 24 carbapenem-resistant E. coli isolates. The distribution of MBL genes in carbapenem-resistant E. coli was as follows: bla(IMP) 18 (72), bla(VIM) 7 (28), and bla(NDM) 1 (4). AmpC was detected in 61.7 (29/47) of the isolates using the phenotypic method. The presence of AmpC genes was confirmed by PCR in only 26 of 29 (86.7) AmpC producers. The frequencies of bla(DHA-1), bla(ACC), and bla(CMY-2) were 6 (20.7), 11 (37.9), and 21 (72.4), respectively. Conclusions The emergence of MBL and AmpC coproducing E. coli isolates calls for an urgent surveillance program for timely diagnosis and screening of these genes in our healthcare systems.

Item Type: Article
Creators:
CreatorsEmail
Bahramian, A.UNSPECIFIED
Khoshnood, S.UNSPECIFIED
Hashemi, N.UNSPECIFIED
Moradi, M.UNSPECIFIED
Karimi-Yazdi, M.UNSPECIFIED
Jalallou, N.UNSPECIFIED
Saki, M.UNSPECIFIED
Keywords: AmpC Escherichia coli Hemodialysis patients Metallo-beta-lactamases Urinary tract infections acinetobacter-baumannii pseudomonas-aeruginosa klebsiella-pneumoniae extended-spectrum prevalence resistance genes sensitivity failure Biochemistry & Molecular Biology
Divisions:
Page Range: pp. 7883-7892
Journal or Publication Title: Molecular Biology Reports
Journal Index: ISI
Volume: 48
Number: 12
Identification Number: https://doi.org/10.1007/s11033-021-06814-y
ISSN: 0301-4851
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3724

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