Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Engineering a multi-epitope vaccine candidate against Leishmania infantum using comprehensive Immunoinformatics methods

Thu Oct 6 23:05:22 2022

(UNSPECIFIED) Engineering a multi-epitope vaccine candidate against Leishmania infantum using comprehensive Immunoinformatics methods. Biologia. p. 13. ISSN 0006-3088

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Abstract

Visceral leishmaniasis (VL) is a severe disease with particular endemicity in over 80 countries worldwide. There is no approved human vaccine against VL in the market. This study was aimed at designing and evaluation of a multimeric vaccine candidate against Leishmania infantum through utilization of helper T lymphocyte (HTL) and cytotoxic T lymphocyte (CTL) immunodominant proteins from histone H1, KMP11, LACK and LeIF antigens. Top-ranked mouse MHC-I, MHC-II binders and CTL epitopes were predicted and joined together via spacers. Also, a TLR-4 agonist (RS-09 synthetic protein) and His-tag were added to the N- and C-terminal of the vaccine sequence, respectively. The final chimeric vaccine had a length of 184 amino acids with a molecular weight of 18.99 kDa. Physico-chemical features showed a soluble, highly-antigenic and non-allergenic candidate. Secondary and tertiary structures were predicted, and subsequent analyses confirmed the construct stability that was capable to properly interact with TLR-4/MD2 receptor. Immunoinformatics simulation displayed potent stimulation of T cell immune responses, with particular rise in IFN-gamma, upon vaccination with the proposed multi-epitope candidate. In conclusion, immunoinformatics data demonstrated a highly antigenic vaccine candidate in mouse, which could develop considerable levels clearance mechanisms and other components of cellular immune profile, and can be directed for VL prophylactic purposes.

Item Type: Article
Creators:
CreatorsEmail
Shams, M.UNSPECIFIED
Nourmohammadi, H.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Shariatzadeh, S. A.UNSPECIFIED
Asghari, A.UNSPECIFIED
Fatollahzadeh, M.UNSPECIFIED
Irannejad, H.UNSPECIFIED
Keywords: Chimeric vaccine Immunoinformatics Leishmania infantum kinetoplastid membrane protein-11 innate immunity prediction recognition antigenicity stability database web Life Sciences & Biomedicine - Other Topics
Divisions:
Page Range: p. 13
Journal or Publication Title: Biologia
Journal Index: ISI
Identification Number: https://doi.org/10.1007/s11756-021-00934-3
ISSN: 0006-3088
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3720

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