Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Prevalence of bacteriocin genes in Lactobacillus strains isolated from fecal samples of healthy individuals and their inhibitory effect against foodborne pathogens

Thu Aug 18 12:16:56 2022

(2021) Prevalence of bacteriocin genes in Lactobacillus strains isolated from fecal samples of healthy individuals and their inhibitory effect against foodborne pathogens. Iranian Journal of Basic Medical Sciences. pp. 1117-1125. ISSN 20083866 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Objective(s): Foodborne diseases are considered as an important public health issue. The purpose of the current study was to isolate Lactobacillus spp. strains from fecal samples, investigate their antimicrobial properties, and assess the expression of genes encoding bacteriocin in co-culture of Lactobacillus with enteric pathogens. Materials and Methods: Fecal samples of healthy people were collected. Human colon adenocarcinoma cell line Caco-2 was used to examine Lactobacillus strains adherence capacity. Quantitative real-time reverse transcription PCR (qRT-PCR) was used to determine bacteriocin-encoding genes expression in co-culture of the selected Lactobacillus strain with Salmonella, Shigella, and two diarrheagenic Escherichia coli serotypes during 4, 6, and 24 hr of incubation. Results: The selected L. plantarum strain was able to inhibit four foodborne pathogens in both methods. L. plantarum No.14 exhibited the highest ability to adhere to Caco-2 cells. In this study, pln F, sak P, pln I, pln B, and pln J genes of L. plantarum No.14 were upregulated in co-culture of L. plantarum No.14 with diarrheagenic E. coli serotypes. In addition, acd, Lactacin F, sak P, pln J, pln EF, and pln NC8 genes as well as pln NC8 and pln A genes mRNA levels were significantly increased in co-culture of L. plantarum No.14 with Shigella dysenteriae, and Salmonella typhi, respectively, during 24 hrs of incubation. Conclusion: Other studied genes were down-regulated during the incubation time. The selected L. plantarum strains could be served as alternative antimicrobial agents against pathogens which could contaminate foodstuffs and are responsible for human diseases. © 2021 Mashhad University of Medical Sciences. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Darbandi, A.UNSPECIFIED
Ghanavati, R.UNSPECIFIED
Asadi, A.UNSPECIFIED
Mirklantari, S.UNSPECIFIED
Hasannejad-Bibalan, M.UNSPECIFIED
Lohrasbi, V.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Rohani, M.UNSPECIFIED
Talebi, M.UNSPECIFIED
Pourshafie, M. R.UNSPECIFIED
Keywords: Bacteriocin Caco-2 cells Escherichia coli Foodborne disease Gene expression Lactobacillus plantarum
Divisions:
Page Range: pp. 1117-1125
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Journal Index: Scopus
Volume: 24
Number: 8
ISSN: 20083866 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3647

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