Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Efficacy of Capparis spinosa Linn Leaf and Fruit Extracts on Giardia Lambia Cysts In vitro

Tue Aug 16 11:38:48 2022

(2021) Efficacy of Capparis spinosa Linn Leaf and Fruit Extracts on Giardia Lambia Cysts In vitro. Journal of Pharmaceutical Research International. pp. 224-230. ISSN 2456-9119

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Abstract

Aims: Giardia lamblia (G. lamblia) is one of the most common intestinal parasites worldwide. There are some side effects and the reports of parasite resistance to metronidazole as the first line treatment of giardiasis. Therefore, it is essential to discover an effect and safe drug to treat giardiasis. Methodology: In this study, the anti-parasitic activity of hydroalcoholic extracts of Capparis spinosa Linn (C. spinosa) leaves and fruits with different concentrations (4 to 0.125 mg/ml) was assessed against human isolates of the G. lamblia cysts and incubated at 37 degrees C. After staining Giardia cysts with 0.1 eosin, the lethal percentage and 50 lethal concentration (LC50) of fruit and leave C. spinosa L extracts and metronidazole on G. lamblia cysts were calculated after 3, 6, 24 and 48 hours. Results: Anti-giardia activity of fruit and leaf extracts of C. spinosa was different between concentrations and time points (p<0.005). The lethal effect of both C. spinosa L extracts and metronidazole increased significantly in a concentration - and time-dependent response (P<0.0001). The highest lethal percentage of G. lamblia cysts was observed a concentration of 4 mg / ml of fruit (100) and leaf (44) extracts of C. spinosa L after 48 hours, respectively. LC50 values were 0.38 +/- 0.02mg/ml for fruit extract, 2.32 +/- 0.1 mg/ml for leaf extract and 0.53 +/- 0.03 mu g/ml for metronidazole after 48hours. Conclusion: C. spinosa can be effective in eliminating Giardia cyst of contaminated environments and water.

Item Type: Article
Creators:
CreatorsEmail
Mohamadian, A.UNSPECIFIED
Mirzaei, A.UNSPECIFIED
Abbasi, N.UNSPECIFIED
Naserifar, R.UNSPECIFIED
Feizi, J.UNSPECIFIED
Karimi, P.UNSPECIFIED
Khorshidi, A.UNSPECIFIED
Maspi, N.UNSPECIFIED
Abdi, J.UNSPECIFIED
Keywords: Giardia lamblia herbal extract Capparis spinosa L medicinal plants anti-parasitic activity pathogenic Pharmacology & Pharmacy
Divisions:
Page Range: pp. 224-230
Journal or Publication Title: Journal of Pharmaceutical Research International
Journal Index: ISI
Volume: 33
Number: 29A
Identification Number: https://doi.org/10.9734/JPRI/2021/v33i29A31582
ISSN: 2456-9119
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3614

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