Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients' urine

Thu Oct 6 07:57:12 2022

(2018) Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients' urine. Infection Genetics and Evolution. pp. 257-268. ISSN 1567-1348

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Abstract

The diagnosis of leishmaniasis relies mainly on the use of invasive processes, to collect the biological material for detecting Leishmania parasites. Body fluids, which can be collected by non-invasive process, would greatly facilitate the leishmaniasis diagnosis. In the present study, we investigated the potency of urine immunoblotting to diagnose cutaneous and visceral leishmaniasis and we compared with routine molecular methods. A total of 80 samples, including 40 sera and their 40 corresponding urine samples were collected from 37 suspected patients with cutaneous and visceral leishmaniasis, and 3 healthy individuals (as control), in Ilam and Ardabil provinces of Iran. All sera and urine samples were analyzed, using immunoblotting. The confirmation of leishmaniasis infection was performed, using conventional and quantitative PCRs as well as by sequencing the amplicons. Among 37 suspected patients, 23 patients presented cutaneous lesions (CL) and 14 exhibited clinical symptoms reminiscent of visceral leishmaniasis (L. infantum). Among cutaneous patients, 15 were positive for zoonotic cutaneous leishmaniasis (L. major), and eight for anthroponotic cutaneous leishmaniasis (L. tropica). Molecular quantification of Leishmania parasites was performed on sera, urines and cutaneous biopsies of CL and VL patients, demonstrating that parasite load is lower in urines, compared to sera or biopsy. DNA can be detected in 20 out of 23 (86.9) CL urine samples and in 13 out of 14 (92.8) VL urine samples. Immunodetection analysis demonstrates that 22 out of 23 (95.6) sera from CL patients and all patients suspected with VL are positive. For urine samples, 18 out of 23 (78.2) urine of CL patients and 13 out of 14 (92.8) urine of VL patients were positive, using Western blot. Therefore, immunodetection and molecular analysis using urine samples can be used as a diagnostic tool for surveying cutaneous and visceral leishmaniasis.

Item Type: Article
Creators:
CreatorsEmail
Mirzaei, A.UNSPECIFIED
Ahmadipour, F.UNSPECIFIED
Cannet, A.UNSPECIFIED
Marty, P.UNSPECIFIED
Delaunay, P.UNSPECIFIED
Perrin, P.UNSPECIFIED
Dorkeld, F.UNSPECIFIED
Sereno, D.UNSPECIFIED
Akhoundi, M.UNSPECIFIED
Keywords: Leishmaniasis Western blot qPCR Serum Urine western-blot-analysis infantum DNA detection real-time pcr clinical leishmaniasis kala-azar diagnosis antigens dogs samples identification Infectious Diseases
Divisions:
Page Range: pp. 257-268
Journal or Publication Title: Infection Genetics and Evolution
Journal Index: ISI
Volume: 63
Identification Number: https://doi.org/10.1016/j.meegid.2018.05.021
ISSN: 1567-1348
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/35

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