Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Formulation and characterization of a novel cutaneous wound healing ointment by silver nanoparticles containing Citrus lemon leaf: A chemobiological study

Thu Aug 18 12:13:53 2022

(2021) Formulation and characterization of a novel cutaneous wound healing ointment by silver nanoparticles containing Citrus lemon leaf: A chemobiological study. Arabian Journal of Chemistry. ISSN 18785352 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Introduction: Formulating new wound-healing ointments by natural compounds is the first research priority in the developing and developed countries. This study was intended to provide green formulation of Ag-NP ointment containing Citrus lemon leaf aqueous extract and examine its capability of healing cutaneous wounds and its antioxidant and cytotoxicity activities under in vitro and in vivo conditions. Materials and methods: Different techniques, including UV–Vis and FT-IR spectroscopy, were used to characterize Ag-NPs. MTT assay was used to investigate cytotoxicity property of Ag-NPs. Antioxidant activity of Ag-NPs were examined by DPPH in the presence of butylated hydroxytoluene as positive control. Parameters of cutaneous wound healing were measured both histopathologically and biochemically. Results: Clear peak at 429 nm shown by UV–Vis spectroscopy indicated formation of Ag-NPs. In FT-IR spectroscopy, presence of many antioxidant compounds provided an excellent condition to reduce silver in Ag-NPs. FE-SEM and TEM images showed spherical Ag-NPs with an average size of 25.1 nm. The synthesized silver nanoparticles had excellent cell viability on the HUVECs line and indicated this method was nontoxic. Application of Ag-NP ointment improved wound healing parameters significantly (P ≤ 0.01). Ag-NPs reduced wound areas, total cells, neutrophils and lymphocytes significantly (P ≤ 0.01) and increased wound contracture, vessels, hexosamines, hydroxyl proline, hexuronic acid, fibrocytes, fibroblasts and fibrocyte/ fibroblast ratios significantly (P ≤ 0.01). Conclusions: Once our results are verified by clinically experimental studies, Ag-NP ointment can be used as a modern one to treat several types of wounds, especially cutaneous ones, in humans. © 2021

Item Type: Article
Creators:
CreatorsEmail
Abbasi, N.UNSPECIFIED
Ghaneialvar, H.UNSPECIFIED
Moradi, R.UNSPECIFIED
Zangeneh, M. M.UNSPECIFIED
Zangeneh, A.UNSPECIFIED
Keywords: Citrus lemon leaf aqueous extract Cutaneous wound healing Ointment Silver nanoparticles
Divisions:
Journal or Publication Title: Arabian Journal of Chemistry
Journal Index: Scopus
Volume: 14
Number: 7
Identification Number: https://doi.org/10.1016/j.arabjc.2021.103246
ISSN: 18785352 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3487

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