Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Differentiation of human dental pulp stem cells into functional motor neuron: In vitro and ex vivo study

Sun Nov 27 21:12:44 2022

(2021) Differentiation of human dental pulp stem cells into functional motor neuron: In vitro and ex vivo study. Tissue & cell. p. 101542. ISSN 1532-3072 (Electronic) 0040-8166 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/33964606

Abstract

There are several therapeutic options for spinal cord injury (SCI), among these strategies stem cell therapy is a potential treatment. The stem cells based therapies have been investigating in acute phase of clinical trials for promoting spinal repair in humans through replacement of functional neuronal and glial cells. The aim of this study was to evaluate the differentiation of Human Dental Pulp Stem Cells (hDPSCs) into functional motor neuron like cells (MNLCs) and promote neuroregeneration by stimulating local neurogenesis in the adult spinal cord slice culture. The immunocytochemistry analysis demonstrated that hDPSCs were positive for mesenchymal stem cell markers (CD73, CD90 and CD105) and negative for the hematopoietic markers (CD34 and CD45). hDPSCs were induced to neurospheres (via implementing B27, EGF, and bFGF) and then neural stem cells (NSC). The NSC differentiated into MNLCs in two steps: first by Shh and RA and ; then with GDNF and BDNF administration. The NS and the NSC were assessed for Oct4, nestin, Nanog, Sox2 expression while the MNLCs were evaluated by ISLET1, Olig2, and HB9 genes. Our results showed that hDPSC can be differentiated into motor neuron phenotype with expression of the motor neuron genes. The functionality of MNLCs was demonstrated by FM1-43, intracellular calcium ion shift and co- culture with C2C12. We co-cultivated hDPSCs with adult rat spinal slices in vitro. Immunostaining and hoechst assay showed that hDPSCs were able to migrate, proliferate and integrate in both the anterolateral zone and the edges of the spinal slices.

Item Type: Article
Creators:
CreatorsEmail
Darvishi, M.UNSPECIFIED
Hamidabadi, H. G.UNSPECIFIED
Bojnordi, M. N.UNSPECIFIED
Saeednia, S.UNSPECIFIED
Zahiri, M.UNSPECIFIED
Niapour, A.UNSPECIFIED
Alizadeh, R.UNSPECIFIED
Keywords: Co-cultivation Human dental pulp stem cells (hDPSCs) Organotype culture Slice culture
Divisions:
Page Range: p. 101542
Journal or Publication Title: Tissue & cell
Journal Index: Pubmed
Volume: 72
Identification Number: https://doi.org/10.1016/j.tice.2021.101542
ISSN: 1532-3072 (Electronic) 0040-8166 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3480

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