Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Comparison of toxin-antitoxin expression among drug-susceptible and drug-resistant clinical isolates of Mycobacterium tuberculosis

Sun Nov 27 22:13:46 2022

(2021) Comparison of toxin-antitoxin expression among drug-susceptible and drug-resistant clinical isolates of Mycobacterium tuberculosis. Advances in Respiratory Medicine. pp. 110-114. ISSN 2451-4934

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Abstract

Introduction: Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB), is a significant global public health threat. Besides extensive multidrug resistance, MTB possesses several properties for long-term viability in the host as well as stress adaptation and resistance in harsh conditions. The role of toxin-antitoxin (TA) systems in disseminating and maintaining antimicrobial resistance in bacterial populations has also been demonstrated. This study aimed to evaluate differences in expression of MazEF (a well-known TA system) related genes (mazE3, mazF3, mazE6, and mazF6) amongst drug-susceptible and resistant MTB isolates in Iran. Material and methods: A total of 20 confirmed clinical isolates of MTB including 10 drug-susceptible and 10 drug-resistant (nine MDR, and one XDR) species were included in this study. M. tuberculosis H37Rv was used as the standard strain. RNA extraction, cDNA synthesis, and relative quantitative real-time PCR were performed according to the standard procedures. Results: Our analysis indicated significant enhanced expression of the mazE6 antitoxin gene in drug-susceptible isolates compared to drug-resistant isolates and the standard strain. The expression of the mazF6 toxin gene was also increased in drug-susceptible isolates compared with the standard strain. In drug-resistant isolates, the expression levels of mazF3 and mazF6 genes were significantly higher than that in the susceptible isolates and the standard strain. Conclusions: In this study, there was significant overexpression of mazE6 in drug-susceptible isolates. As well, mazF3 and F6 were overexpressed in drug-resistant isolates when compared with the standard strain. The changes in expression levels of MazEF6 associated genes were greater than that of MazEF3 in both groups of isolates.

Item Type: Article
Creators:
CreatorsEmail
Kazemian, H.UNSPECIFIED
Heidari, H.UNSPECIFIED
Kardan-Yamchi, J.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Kouhsari, E.UNSPECIFIED
Valadbeigi, H.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Keywords: Mycobacterium tuberculosis toxin-antitoxin system MazEF gene expression systems mazef Respiratory System
Divisions:
Page Range: pp. 110-114
Journal or Publication Title: Advances in Respiratory Medicine
Journal Index: ISI
Volume: 89
Number: 2
Identification Number: https://doi.org/10.5603/ARM.a2021.0033
ISSN: 2451-4934
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3465

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