Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Expression of type II toxin-antitoxin systems and ClpP protease of methicillin-resistant Staphylococcus aureus under thermal and oxidative stress conditions

Tue Aug 16 05:20:48 2022

(2021) Expression of type II toxin-antitoxin systems and ClpP protease of methicillin-resistant Staphylococcus aureus under thermal and oxidative stress conditions. Iranian Journal of Microbiology. pp. 204-211. ISSN 2008-3289

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Abstract

Background and Objectives: Staphylococcus aureus is a main human pathogen that causes a variety of chronic to persistent infections. Across the diverse factors of pathogenesis in bacteria, Toxin-Antitoxin (TA) systems can be considered as an antibacterial target due to their involvement in cellular physiology counting stress responses. Here, the expression of TA system genes and ClpP protease was investigated under the thermal and oxidative conditions in S. aureus strains. Materials and Methods: The colony-forming unit (CFU) was used to determine the effects of thermal and oxidative stresses on bacterial survival. Moreover, the expressions of TA system genes in S. aureus strains were evaluated 30 min and 1 h after thermal and oxidative stresses, respectively, by quantitative reverse transcriptase real-time PCR (qRT-PCR). Results: The cell viability was constant across thermal stress while oxidative stress induction showed a significantly decrease in the growth of Methicillin-Resistant S. aureus (MRSA) strain. Based on the qRT-PCR results, the expression of mazF gene increased under both thermal and oxidative stresses in the MRSA strain. Conclusion: A putative TA system (namely immA/irrA) most likely has a role under the stress condition of S. aureus. The MRSA strain responds to stress by shifting the expression level of TA genes that has diverse effects on the survival of the pathogen due to the stress conditions. The TA systems may be introduced as potential targets for antibacterial treatment.

Item Type: Article
Creators:
CreatorsEmail
Karimaei, S.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Shahrokhi, N.UNSPECIFIED
Mashhadi, R.UNSPECIFIED
Pourmand, M. R.UNSPECIFIED
Keywords: Staphylococcus aureus Oxidative stress Toxin-antitoxin systems Cold-shock response Heat-shock response heat-shock Microbiology
Divisions:
Page Range: pp. 204-211
Journal or Publication Title: Iranian Journal of Microbiology
Journal Index: ISI
Volume: 13
Number: 2
ISSN: 2008-3289
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3458

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