Repository of Research and Investigative Information

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Ilam University of Medical Sciences

Identification of Trichomonas Vaginalis Genotypes Using by Actin Gene and Molecular Based Methods in Southwest of Iran

Tue Aug 16 12:29:48 2022

(2021) Identification of Trichomonas Vaginalis Genotypes Using by Actin Gene and Molecular Based Methods in Southwest of Iran. Reports of Biochemistry and Molecular Biology. pp. 135-143. ISSN 2322-3480

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Abstract

Background: Trichomonas vaginalis (T. vaginalis) is a sexually transmitted protozoan parasite and the causative agent of trichomoniasis. The genetic characterization of T. vaginalis isolates shows notable genetic variation in this parasite. In the present study, we aimed to identify the T. vaginalis genotypes based on analyzing of actin gene in women specimens referred to health centers of Ilam city, southwest Iran. Methods: A total of 1765 female samples were collected from gynecology clinics in the city of Ilam. DNA was extracted from positive samples and nested polymerase chain reaction (Nested PCR) was used to amplify the actin gene. Then, partial sequencing and genotyping of the actin gene was performed. A phylogenetic tree was drawn using the detected genotypes of T. vaginalis and reference sequences. Results: Twenty-one of the 1765 urine and vaginal samples were positive for T. vaginalis. All infected individuals were married and their age in years was between 25 to 34. Further, the majority of infected women had cervical lesions, patchy erythema, and white color discharge. According to sequencing analysis, the isolates were identified as genotype G (n = 8) and genotype E (n = 2). Conclusions: From the collected samples, we were able to distinguish at least two genotypes (G and E) of T. vaginalis. However, lesser is known about these genotypes in the city of Ilam. Further studies with a higher number of isolates should be performed in order to understand the implications of these results in this region.

Item Type: Article
Creators:
CreatorsEmail
Alikhani, M.UNSPECIFIED
Saberi, R.UNSPECIFIED
Hosseini, S. A.UNSPECIFIED
Rezaei, F.UNSPECIFIED
Pagheh, A. S.UNSPECIFIED
Mirzaei, A.UNSPECIFIED
Keywords: Actin gene Genotypes Ilam Iran Trichomonas vaginalis clinical-manifestations pcr women diversity population prevalence sequence genome Biochemistry & Molecular Biology
Divisions:
Page Range: pp. 135-143
Journal or Publication Title: Reports of Biochemistry and Molecular Biology
Journal Index: ISI
Volume: 10
Number: 1
ISSN: 2322-3480
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3455

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