Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Di-(2-ethylhexyl) Phthalate-Induced Hippocampus-Derived Neural Stem Cells Proliferation

Tue Jul 23 02:57:03 2024

(2017) Di-(2-ethylhexyl) Phthalate-Induced Hippocampus-Derived Neural Stem Cells Proliferation. Cell Journal. pp. 166-172. ISSN 2228-5806

Full text not available from this repository.

Official URL:


The brain and spinal cord have a limited capacity for self-repair under damaged conditions. One of the best options to overcome these limitations involves the use of phytochemicals as potential therapeutic agents. In this study, we have aimed to investigate the effects of di-(2-ethylhexyl) phthalate (DEHP) on hippocampus-derived neural stem cells (NSCs) proliferation to search phytochemical candidates for possible treatment of neurological diseases using endogenous capacity. In this experimental study, neonatal rat hippocampus-derived NSCs were cultured and treated with various concentrations of DEHP (0, 100, 200, 400 and 600 mu M) and Cirsium vulgare (C. vulgare) hydroethanolic extract (0, 200, 400, 600, 800 and 1000 mu g/ml) for 48 hours under in vitro conditions. Cell proliferation rates and quantitative Sox2 gene expression were evaluated using MTT assay and real-time reverse transcription polymerase chain reaction (RT-PCR). We observed the highest average growth rate in the 400 mu M DEHP and 800 mu g/ml C. vulgare extract treated groups. Sox2 expression in the DEHP-treated NSCs significantly increased compared to the control group. Gas chromatography/mass spectrometry (GC/MS) results demonstrated that the active ingredients that naturally occurred in the C. vulgare hydroethanolic extract were 2-ethyl-1-hexanamine, n-heptacosane, 1-cyclopentane-carboxylic acid, 1-heptadecanamine, 2,6-octadien-1-ol, 2,6,10,14,18,22-tetracosahexaene, and DEHP. DEHP profoundly stimulated NSCs proliferation through Sox2 gene overexpression. These results provide and opportunity for further use of the C. vulgure phytochemicals for prevention and/or treatment of neurological diseases via phytochemical mediated-proliferation of endogenous adult NSCs.

Item Type: Article
Mesbah-Namin, S. A.UNSPECIFIED
Nejatbakhsh, R.UNSPECIFIED
Keywords: Proliferation Sox2 Neural Stem Cells neurological disorders subventricular zone multiple-sclerosis self-renewal human brain neurogenesis model multipotent striatum protects Cell Biology
Page Range: pp. 166-172
Journal or Publication Title: Cell Journal
Journal Index: ISI
Volume: 19
Number: 1
ISSN: 2228-5806
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item