Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The 7H11 Agar Medium Supplemented with Calf Bovine Serum for Susceptibility Testing of Mycobacterium tuberculosis Isolates Against Pyrazinamide

Sun Nov 27 21:56:38 2022

(2021) The 7H11 Agar Medium Supplemented with Calf Bovine Serum for Susceptibility Testing of Mycobacterium tuberculosis Isolates Against Pyrazinamide. Microbial Drug Resistance. p. 6. ISSN 1076-6294

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Abstract

Despite its importance, pyrazinamide (PZA) is a blind spot in drug susceptibility testing in tuberculosis laboratories. The aim of this study was to set up a reliable agar-based proportion method for detection of PZA-resistant phenotypes using Middlebrook 7H11 agar supplemented with calf bovine serum (CBS) compared with albumin/dextrose/catalase (ADC) enrichment and pncA/rpsA sequencing results. The 7H11 agar medium supplemented with 10 ADC or 10 CBS (pH 6.2) and 100 mu g/mL PZA was used to detect PZA resistance among 64 Mycobacterium tuberculosis isolates. Sanger sequencing and whole-genome sequencing were performed to track mutations in the pncA, rpsA, and their upstream regions. A total of 43 rifampicin/multidrug-resistant, 20 drug-susceptible, and 1 isoniazid mono-resistant M. tuberculosis isolates were investigated. The 7H11+ADC and 7H11+CBS could detect 22 and 23 PZA-resistant strains, respectively. With the same specificity, the sensitivity and accuracy of 7H11+CBS was found to be a little greater than 7H11+ADC in PZA resistance detection compared with sequencing results. Twenty-four mutant strains were found to have different mutations in pncA-upstream, pncA and rpsA genes, in which Gly97Asp was the most dominant mutation. The results obtained from 7H11+CBS were comparable to the results of 7H11+ADC. Therefore, the 7H11 agar proportion method would be a less-expensive test using CBS and produces reliable results.

Item Type: Article
Creators:
CreatorsEmail
Amini, S.UNSPECIFIED
Kardan-Yamchi, J.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Nasiri, M. J.UNSPECIFIED
Hamzehloo, G.UNSPECIFIED
Hoffner, S.UNSPECIFIED
Feizabadi, M. M.UNSPECIFIED
Keywords: Mycobacterium tuberculosis pyrazinamide drug resistance calf bovine serum pncA drug-resistance Infectious Diseases Microbiology Pharmacology & Pharmacy
Divisions:
Page Range: p. 6
Journal or Publication Title: Microbial Drug Resistance
Journal Index: ISI
Identification Number: https://doi.org/10.1089/mdr.2020.0509
ISSN: 1076-6294
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3436

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