Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Therapeutic effects, immunogenicity and cytotoxicity of a cell penetrating peptide-peptide nucleic acid conjugate against cagA of Helicobacter pylori in cell culture and animal model

Sun Nov 27 23:10:36 2022

(2021) Therapeutic effects, immunogenicity and cytotoxicity of a cell penetrating peptide-peptide nucleic acid conjugate against cagA of Helicobacter pylori in cell culture and animal model. Iranian Journal of Microbiology. pp. 360-371. ISSN 2008-3289

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Abstract

Background and Objectives: Helicobacter pylori causes several gastrointestinal diseases, including asymptomatic gastritis, chronic peptic ulcer, duodenal ulcer, lymphoma of the mucosa-associated lymphoid tissue (MALT), and gastric adenocarcinoma. In recent years, failure to eradicate H. pylori infections has become an alarming problem for physicians. It is now clear that the current treatment strategies may become ineffective, necessitating the development of innovative antimicrobial compounds as alternative treatments. Materials and Methods: In this experimental study, a cell-penetrating peptide-conjugated peptide nucleic acid (CPP-PNA) was used to target the cagA expression. cagA expression was evaluated using RT-qPCR assay after treatment by the CPPPNA in cell culture and animal model. Additionally, immunogenicity and toxicity of the CPP-PNA were assessed in both cell culture and animal models. Results: Our analysis showed that cagA mRNA levels reduced in H. pylori-infected HT29 cells after treatment with CPP-PNA in a dose-dependent manner. Also, cagA expression in bacterial RNA extracted from stomach tissue of mice treated with PNA was reduced compared to that of untreated mice. The expression of inflammatory cytokines also decreased in cells and tissue of H. pylori-infected mice after PNA treatment. The tested CPP-PNA showed no significant adverse effects on cell proliferation of cultured cells and no detectable toxicity and immunogenicity were observed in mice. Conclusion: These results suggest the effectiveness of CPP-PNA in targeting CagA for various research and therapeutic purposes, offering a potential antisense therapy against H. pylori infections.

Item Type: Article
Creators:
CreatorsEmail
Farahani, N. N.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Monavar, S. H.UNSPECIFIED
Mirkalantari, S.UNSPECIFIED
Montazer, F.UNSPECIFIED
Sholeh, M.UNSPECIFIED
Javanmard, Z.UNSPECIFIED
Irajian, G.UNSPECIFIED
Keywords: Helicobacter pylori cag A Peptide nucleic acid Immunogenicity Cytotoxicity time quantitative pcr antisense oligonucleotides tyrosine phosphorylation virulence inhibition expression therapies infection prospects health Microbiology
Divisions:
Page Range: pp. 360-371
Journal or Publication Title: Iranian Journal of Microbiology
Journal Index: ISI
Volume: 13
Number: 3
ISSN: 2008-3289
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3435

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