Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Development of a chimeric vaccine candidate based on Toxoplasma gondii major surface antigen 1 and apicoplast proteins using comprehensive immunoinformatics approaches

Mon Oct 3 05:29:06 2022

(2021) Development of a chimeric vaccine candidate based on Toxoplasma gondii major surface antigen 1 and apicoplast proteins using comprehensive immunoinformatics approaches. European Journal of Pharmaceutical Sciences. p. 12. ISSN 0928-0987

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Abstract

This study was aimed at designing and evaluation of a multimeric vaccine construct against Toxoplasma gondii via utilization of SAG1 along with apicoplast ribosomal proteins (S2, S5 and L11). Top-ranked MHC-I and MHC-II binding as well as shared, immunodominant linear B-cell epitopes were predicted and joined together via appropriate linkers. Also, TLR-4 agonist (RS-09 synthetic protein) and His-tag were added to the N- and C-terminal of the vaccine sequence. The finally-engineered chimeric vaccine had a length of 291 amino acids with a molecular weight of 31.46 kDa. Physico-chemical features showed a soluble, highly-antigenic and non-allergenic candidate. Secondary and tertiary structures were predicted, and subsequent analyses confirmed the construct stability that was capable to properly interact with human TLR-4. Immunoinformatics-based simulation displayed potent stimulation of T- and B-cell mediated immune responses upon vaccination with the proposed multi-epitope candidate. In conclusion, obtained information demonstrated a highly antigenic vaccine candidate, which could develop high levels of IFN-gamma and other components of cellular immune profile, and can be directed for toxoplasmosis prophylactic purposes.

Item Type: Article
Creators:
CreatorsEmail
Asghari, A.UNSPECIFIED
Shamsinia, S.UNSPECIFIED
Nourmohammadi, H.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Fatollahzadeh, M.UNSPECIFIED
Nemati, T.UNSPECIFIED
Irannejad, H.UNSPECIFIED
Nouri, H. R.UNSPECIFIED
Ghasemi, E.UNSPECIFIED
Shams, M.UNSPECIFIED
Keywords: Chimeric vaccine Immunoinformatics Toxoplasma gondii b-cell epitopes prediction tool immunology linkers design web Pharmacology & Pharmacy
Divisions:
Page Range: p. 12
Journal or Publication Title: European Journal of Pharmaceutical Sciences
Journal Index: ISI
Volume: 162
Identification Number: https://doi.org/10.1016/j.ejps.2021.105837
ISSN: 0928-0987
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3429

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