Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Genotyping and drug susceptibility patterns of M. tuberculosis isolated from HIV seropositive patients in Tehran Iran

Sun Nov 27 22:08:34 2022

(2021) Genotyping and drug susceptibility patterns of M. tuberculosis isolated from HIV seropositive patients in Tehran Iran. Current HIV research. ISSN 1873-4251 (Electronic) 1570-162X (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/33634760

Abstract

AIM: to investigate the prevalence and drug-resistance M. tuberculosis isolated from HIV seropositive individuals in Tehran, Iran. BACKGROUND: Human immunodeficiency virus (HIV) is one of the most important risk factors for developing active tuberculosis (TB). OBJECTIVE: To determine the rate of transmission and drug resistant M. tuberculosis (MTB) strains isolated from HIV seropositive patients in Tehran province, Iran. METHODS: This study consecutively enrolled 217 TB/HIV coinfected patients from April 2018 to August 2019 at Emam Khomeini referral hospital and 5 other health centers in Tehran province. The isolates were genotyped using 15 loci Mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR). Minimum inhibitory concentration (MIC) was determined for 6 drugs. In addition, mutations were assessed in rpoB, katG, inhA, and ahpC genes using Reverse Blot Hybridization Assay System. RESULTS: A 20 (9.2) patients were culture-positive for M. tuberculosis and typed by MIRU-VNTR, 13 (65) strains formed 5 clusters, but 6 (30) isolates had a unique pattern. The total Hunter-Gaston discrimination index (HGDI) for all 15 loci was 0.846, and the cluster size was 2 to 4 patients. The estimated proportion of recent transmission was 45. The mutation was identified in 1 isolate, lost inhAW1 and mutation in MT1 loci, which was resistant to isoniazid (INH). Moreover, 1 (5) and 3 (15) isolates were resistant to INH and ethambutol (EMB), respectively, of which 1 was resistant to INH and EMB. CONCLUSION: The transmission rate of TB in HIV patients was relatively high; however, the prevalence of drug-resistant strains and TB infection in females was insignificant in this study (p < 0.05); none of the isolates was MDR strains.

Item Type: Article
Creators:
CreatorsEmail
Feyisa, S. G.UNSPECIFIED
Rasoolinejad, M.UNSPECIFIED
Hasan-Nejad, M.UNSPECIFIED
Amini, S.UNSPECIFIED
Hamzelou, G.UNSPECIFIED
Kardan-Yamchi, J.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Karami-Zarandi, M.UNSPECIFIED
Feizabadi, M. M.UNSPECIFIED
Keywords: Cluster Hiv M. tuberculosis Miru-vntr Minimum Inhibitory Concentration Mutation Reverse Blot Hybridization
Divisions:
Journal or Publication Title: Current HIV research
Journal Index: Pubmed
Identification Number: https://doi.org/10.2174/1570162X19666210226125335
ISSN: 1873-4251 (Electronic) 1570-162X (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3385

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